Reduction in chromosome mobility accompanies nuclear organization during early embryogenesis in Caenorhabditis elegans
| Autor: | Arai, R., Sugawara, T., Minakuchi, Y., Sato, Yuko, Toyoda, A., Nabeshima, K., Kimura, Hiroshi, Kimura, A. |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Nucleolus Heterochromatin Cellular differentiation Science Embryonic Development Biology Chromosomes Article 03 medical and health sciences 0302 clinical medicine Homologous chromosome medicine Animals Caenorhabditis elegans Genetics Multidisciplinary Genome Whole Genome Sequencing Chromosome biology.organism_classification Cell biology Molecular Imaging Cell nucleus 030104 developmental biology medicine.anatomical_structure Medicine Interphase 030217 neurology & neurosurgery |
| Zdroj: | Scientific Reports Scientific Reports, Vol 7, Iss 1, Pp 1-10 (2017) |
| ISSN: | 2045-2322 |
| Popis: | In differentiated cells, chromosomes are packed inside the cell nucleus in an organised fashion. In contrast, little is known about how chromosomes are packed in undifferentiated cells and how nuclear organization changes during development. To assess changes in nuclear organization during the earliest stages of development, we quantified the mobility of a pair of homologous chromosomal loci in the interphase nuclei of Caenorhabditis elegans embryos. The distribution of distances between homologous loci was consistent with a random distribution up to the 8-cell stage but not at later stages. The mobility of the loci was significantly reduced from the 2-cell to the 48-cell stage. Nuclear foci corresponding to epigenetic marks as well as heterochromatin and the nucleolus also appeared around the 8-cell stage. We propose that the earliest global transformation in nuclear organization occurs at the 8-cell stage during C. elegans embryogenesis. |
| Databáze: | OpenAIRE |
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