Development and validation a liquid chromatography mass spectrometry for determination of solasodine in rat plasma and its application to a pharmacokinetic study
| Autor: | Jianshe Ma, Xianqin Wang, Chongliang Lin, Guanyang Lin, Xitao Ding, Xuezhi Yang, Chengxiang Sun, Xinxin An |
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| Rok vydání: | 2014 |
| Předmět: |
Male
Spectrometry Mass Electrospray Ionization Chromatography Formic acid Electrospray ionization Clinical Biochemistry Extraction (chemistry) Ethyl acetate Administration Oral Cell Biology General Medicine Mass spectrometry Solanaceous Alkaloids Biochemistry Solasodine Analytical Chemistry Rats Sprague-Dawley chemistry.chemical_compound chemistry Limit of Detection Liquid chromatography–mass spectrometry Animals Sample preparation Chromatography Liquid |
| Zdroj: | Journal of Chromatography B. 963:24-28 |
| ISSN: | 1570-0232 |
| DOI: | 10.1016/j.jchromb.2014.05.031 |
| Popis: | Solasodine is a poisonous alkaloid chemical compound that occurs in plants of the Solanaceae family. A simple and selective liquid chromatography mass spectrometry method for determination of solasodine in rat plasma was developed and validated over the range of 3-1,000 ng/mL. Chromatographic separation was achieved on a C18 (2.1 mm×50 mm, 3.5 μm) column with acetonitrile-0.1% formic acid in water as mobile phase with gradient elution. The flow rate was set at 0.4 mL/min. After addition of midazolam as internal standard (IS), liquid-liquid extraction by ethyl acetate was used as sample preparation. An electrospray ionization source was applied and operated in positive ion mode; selective ion monitoring mode was used for quantification with target ions m/z 414 for solasodine and m/z 326 for IS. Mean recoveries of solasodine in rat plasma were in the range of 87.6-94.1%. Matrix effects for solasodine were between 94.9% and 102.3%. Coefficient of variation of intra-day and inter-day precision were both |
| Databáze: | OpenAIRE |
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