Characterization of a T cell-derived lymphokine that acts synergistically with IL 3 on the growth of murine mast cells and is identical with IL4
Autor: | R. Schwarzkopf, Eberhard Spaeth, K. Beyreuther, Edgar Schmitt, Erwin Rüde, Birgit Fassbender |
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Rok vydání: | 1987 |
Předmět: |
T-Lymphocytes
medicine.medical_treatment T cell Immunology Biology Cell Line Mice medicine Animals Immunology and Allergy Mast Cells Interleukin 4 Interleukin 3 Interleukins Growth factor Lymphokine Antibodies Monoclonal Hematology Mast cell Molecular biology Recombinant Proteins Molecular Weight Cytokine medicine.anatomical_structure Cell culture Electrophoresis Polyacrylamide Gel Interleukin-3 Interleukin-4 |
Zdroj: | Immunobiology. 174:406-419 |
ISSN: | 0171-2985 |
DOI: | 10.1016/s0171-2985(87)80014-1 |
Popis: | A mast cell-like cell line (SN-1) was established with the aid of growth factor(s) present in the supernatant of a Con A-stimulated L3T4 + T cell line. In analogy to other mast cell lines, IL 3 was identified as a growth factor for SN-1 cells. In addition, a second lymphokine produced by the T cells synergistically enhanced the IL 3-induced growth. This factor, originally termed mast cell growth enhancing factor (MaGEF), could be separated from IL 2, IL 3, a CSF-like activity and was purified to homogeneity. The N-terminal amino acid sequence (8 residues) and the functional properties of this lymphokine proved to be identical with those reported for BSF-1 (IL 4). Unless applied at high concentrations, purified MaGEF did not stimulate growth of the SN-1 mast cells in the absence of IL 3. MaGEF was also found to act on two IL 2-dependent T cell lines by inducing significant thymidine incorporation which was suboptimal compared to that induced by IL 2 and which cannot be inhibited by anti-IL 2-antibodies. A panel of cell lines developed from mouse bone marrow with IL 3 or with a combination of IL 3 and MaGEF all reacted to MaGEF in the presence of IL 3 with considerably increased proliferation. It is therefore suggested that one of the physiological functions of MaGEF is to promote the recruitment of T-dependent mast cells. |
Databáze: | OpenAIRE |
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