Single vs. two-photon microscopy for label free intrinsic tissue studies in the UV light region
| Autor: | Franck Chiappini, Frédéric Jamme, Matthieu Réfrégiers, Vitalijs Zubkovs, François Le Naour, Slavka Kascakova |
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| Přispěvatelé: | Ecole Polytechnique Fédérale de Lausanne (EPFL), Synchrotron SOLEIL (SSOLEIL), Centre National de la Recherche Scientifique (CNRS), Physiopathologie et traitement des maladies du foie, Institut National de la Santé et de la Recherche Médicale (INSERM)-Hôpital Paul Brousse-Université Paris-Sud - Paris 11 (UP11), Synchrotron SOLEIL, Refregiers, Matthieu, Université Paris-Sud - Paris 11 (UP11)-Hôpital Paul Brousse-Institut National de la Santé et de la Recherche Médicale (INSERM) |
| Rok vydání: | 2014 |
| Předmět: |
Photon
Materials science Ultraviolet Rays [SDV.BBM.BP] Life Sciences [q-bio]/Biochemistry Molecular Biology/Biophysics Nanotechnology medicine.disease_cause Biochemistry Analytical Chemistry 03 medical and health sciences 0302 clinical medicine Two-photon excitation microscopy Electrochemistry medicine DUV Environmental Chemistry Spectroscopy 030304 developmental biology Label free Microscopy Photons 0303 health sciences [SDV.BBM.BP]Life Sciences [q-bio]/Biochemistry Molecular Biology/Biophysics Rat tail tendon Biophysics 030211 gastroenterology & hepatology Excitation Ultraviolet |
| Zdroj: | Analyst Analyst, Royal Society of Chemistry, 2014, 139, pp.2663-2667. ⟨10.1039/c4an00203b⟩ Analyst, 2014, 139, pp.2663-2667. ⟨10.1039/c4an00203b⟩ |
| ISSN: | 1364-5528 0003-2654 |
| DOI: | 10.1039/c4an00203b |
| Popis: | International audience; Fibrillar distribution in the rat tail tendon and mice liver can be measured using optical methods. Two-photon excitation provides easy assessment of fibrotic collagen types I and II. Single photon deep ultraviolet (DUV) excitation imaging highlights all collagen types without discrimination. Their combination on the same tissue area provides a better overview of collagens in fibrillar diseases. |
| Databáze: | OpenAIRE |
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