Extracellular matrix constituents affect superficial gastric epithelial cell adhesion
| Autor: | E. R. Lacy, D. Revis, D. Rhodes |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Pathology
medicine.medical_specialty Guinea Pigs Epithelium Type IV collagen Laminin medicine Gastric mucosa Cell Adhesion Animals Cycloheximide Cells Cultured Matrigel Extracellular Matrix Proteins Hepatology biology business.industry Gastroenterology Cell biology Fibronectins Fibronectin Foveolar cell Drug Combinations medicine.anatomical_structure Gastric pits Gastric Mucosa biology.protein Basal lamina Female Proteoglycans Collagen business |
| Zdroj: | Journal of gastroenterology and hepatology. 9 |
| ISSN: | 0815-9319 |
| Popis: | The interfoveolar and upper gastric pit cells become necrotic and slough off after superficial luminal injury to the gastric mucosa. The subsequent rapid epithelial restitution of the wound is dependent on an intact basal lamina upon which viable mucous cells migrate. Several lines of evidence suggest that migrating mucous cells recognize specific moieties in the basal lamina which would then affect restitution and the ability of the gastric mucosa to be repaired. Therefore, this study examined the effect of three individual protein constituents of the extracellular matrix, laminin, fibronectin and type IV collagen as well as a synthetic basal lamina, Matrigel, on adherence of mucous cells isolated from guinea-pig stomach to these substrates in culture. After 3 h, approximately 40% of the cells adhered to Matrigel, 25% to both collagen IV and fibronectin, but only about 10% to laminin and 3% to uncoated plastic substrates. Disruption of protein synthesis by pre-incubation with cyclohexamide significantly reduced adherence to Matrigel and collagen IV but not laminin, fibronectin or plastic substrates. These results suggest that gastric mucous cells have multiple receptors for extracellular matrix proteins (ligands) which influence the adherence and probably the migration of these cells. Furthermore, some of these receptors are synthesized in response to moieties in the substrate itself. |
| Databáze: | OpenAIRE |
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