Detection of tumor DNA in serum of colorectal cancer patients
| Autor: | Johannes L. Willems, R. W. H. M. Roelofs, G.N.P. van Muijen, J.B. de Kok, W. W. Van Solinge, Dorine W. Swinkels, Theo J.M. Ruers |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1997 |
| Předmět: |
Pathology
medicine.medical_specialty Colorectal cancer Diagnostiek van maag-darm pathologie door middel van faeces onderzoek medicine.medical_treatment DNA Mutational Analysis Clinical Biochemistry Rectum Angiogenesis Factor Polymerase Chain Reaction Laboratory chemistry.chemical_compound Neoplasms Diagnosis Econometric and Statistical Methods: Special Topics Preventive Health Services medicine Humans Mass Screening Point Mutation Tumor pathology Neoplasm Metastasis Lung cancer GeneralLiterature_REFERENCE(e.g. dictionaries encyclopedias glossaries) Gene Chemotherapy business.industry Point mutation Head and neck cancer Special Topics [Econometric and Statistical Methods] DNA Neoplasm General Medicine Chirurgische Oncologie Tumor pathologie medicine.disease Diagnosis Laboratory Detection of pathology of the intestinal tract by means of investigation of the stool Genes ras medicine.anatomical_structure Surgical Oncology chemistry Cancer research Colorectal Neoplasms business DNA |
| Zdroj: | Scandinavian Journal of Clinical and Laboratory Investigation, 57, pp. 601-604 Scandinavian Journal of Clinical and Laboratory Investigation, 57, 11, pp. 601-604 Scandinavian Journal of Clinical and Laboratory Investigation, 57, 601-604 |
| ISSN: | 0036-5513 |
| Popis: | Circulating tumour DNA has previously been detected in serum and plasma of patients with lung cancer and head and neck cancer. These observations could potentially lead to new, specific and non-invasive tools for diagnosis, prognosis and follow-up in neoplastic disease, if found to be a more general phenomenon. To test if tumour DNA is also present in serum of patients with colorectal cancer, we selected 14 colorectal cancer patients with advanced disease. In seven patients, K-ras mutations were detected in the primary tumour, using mutant-specific primers for point mutations in codon 12 or 13 of the K-ras gene. All patients were analysed for mutant DNA in serum. Tumour-specific point mutations, corresponding to the K-ras mutations found in the primary tumour were detected in the serum of all patients but one. No mutant K-ras could be detected in the serum of seven patients without K-ras mutations in the primary tumour. These results may be useful in assessing tumour burden in patients with neoplastic disease. Moreover, consecutive testing of serum tumour DNA after surgery or chemotherapy may be used as a tumour marker for recurrent disease. |
| Databáze: | OpenAIRE |
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