Developing a pathway-independent and full-autonomous global resource allocation strategy to dynamically switching phenotypic states
| Autor: | Jiahua Gao, Bao Meijiao, Shiyao Cheng, Junjun Wu, Qianqian Zhuang, Xuguo Duan, Caiwen Chen, Peng Zhou, Zhijun Zhao |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Computer science Distributed computing Science RNA Stability General Physics and Astronomy MRNA Decay 01 natural sciences General Biochemistry Genetics and Molecular Biology Article 03 medical and health sciences 010608 biotechnology Escherichia coli Production (economics) lcsh:Science Synthetic biology Multidisciplinary Escherichia coli Proteins Fatty Acids Quorum Sensing General Chemistry Gene Expression Regulation Bacterial Phenotype Biosynthetic Pathways Quorum sensing 030104 developmental biology Metabolic Engineering Fermentation Biocatalysis Resource allocation lcsh:Q Flux (metabolism) Host (network) Dynamic resource |
| Zdroj: | Nature Communications, Vol 11, Iss 1, Pp 1-14 (2020) Nature Communications |
| ISSN: | 2041-1723 |
| Popis: | A grand challenge of biological chemical production is the competition between synthetic circuits and host genes for limited cellular resources. Quorum sensing (QS)-based dynamic pathway regulations provide a pathway-independent way to rebalance metabolic flux over the course of the fermentation. Most cases, however, these pathway-independent strategies only have capacity for a single QS circuit functional in one cell. Furthermore, current dynamic regulations mainly provide localized control of metabolic flux. Here, with the aid of engineering synthetic orthogonal quorum-related circuits and global mRNA decay, we report a pathway-independent dynamic resource allocation strategy, which allows us to independently controlling two different phenotypic states to globally redistribute cellular resources toward synthetic circuits. The strategy which could pathway-independently and globally self-regulate two desired cell phenotypes including growth and production phenotypes could totally eliminate the need for human supervision of the entire fermentation. A challenge for biological chemical production is the completion between synthetic circuits and host resources. Here the authors the authors use quorum sensing circuits and global mRNA decay to independently control two phenotypic states. |
| Databáze: | OpenAIRE |
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