Emergence of a colistin-resistant KPC-2-producing Klebsiella pneumoniae ST258 clone in Hungary
| Autor: | K. Böröcz, Ákos Tóth, A. Dobák, E. Puskás, Laura Jánvári, Judit Pászti, M. Farkas, Ivelina Damjanova |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Adult
DNA Bacterial Male Microbiology (medical) Genotype Klebsiella pneumoniae Microbial Sensitivity Tests Polymerase Chain Reaction beta-Lactamases Microbiology law.invention Bacterial Proteins law Drug Resistance Multiple Bacterial Pulsed-field gel electrophoresis medicine Cluster Analysis Humans Etest Polymerase chain reaction Aged Antibacterial agent Aged 80 and over Hungary biology Colistin General Medicine Middle Aged biochemical phenomena metabolism and nutrition bacterial infections and mycoses biology.organism_classification DNA Fingerprinting Anti-Bacterial Agents Bacterial Typing Techniques Electrophoresis Gel Pulsed-Field Klebsiella Infections Infectious Diseases Carbapenems DNA profiling Multilocus sequence typing Female medicine.drug |
| Zdroj: | European Journal of Clinical Microbiology & Infectious Diseases. 29:765-769 |
| ISSN: | 1435-4373 0934-9723 |
| Popis: | Nine Klebsiella pneumoniae isolates showing non-susceptibility to carbapenems were collected from three centres in the north-eastern region of Hungary. The minimum inhibitory concentrations (MICs) of antibiotics were determined by Etest. The putative production of a carbapenemase was tested by the modified Hodge test. The presence of bla (KPC) genes was verified by polymerase chain reaction (PCR) and sequencing. Furthermore, molecular typing was performed by pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). All isolates showed extensively drug-resistant (XDR) phenotype, and of these, eight isolates were highly resistant to colistin. The isolates carried bla (KPC-2), bla (SHV-12), bla (TEM-1) and bla (SHV-11). PFGE analysis of the nine KPC-2-producing Hungarian ST258 K. pneumoniae isolates, two KPC-2-producing Norwegian ST258 isolates and 33 CTX-M-15-producing ST11 isolates revealed the existence of one genetic cluster at an 88% similarity level. The overall results of the PFGE clustering, MLST and the presence of SHV-11 in both ST11 and ST258 suggest that this is the first hyperepidemic clonal complex of multidrug-resistant K. pneumoniae, probably CC258/CC340, possibly undergoing worldwide spread. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink