Gene and protein expression in response to different growth temperatures and oxygen availability in Burkholderia thailandensis

Autor: Stephen L. Michell, Luca Freddi, Paolo Landini, Pierluigi Mauri, Clarissa Consolandi, Alessandro Pietrelli, Sébastien Jaillon, Sara Motta, Fabrizio Basilico, Olivia L. Champion, Fabrizio Chiaramonte, Gianluca De Bellis, Luigi Falciola, Clelia Peano, Cecilia Garlanda, Elio Rossi
Jazyk: angličtina
Rok vydání: 2014
Předmět:
Proteomics
Lipopolysaccharides
Time Factors
Neutrophils
ved/biology.organism_classification_rank.species
Gene Expression
lcsh:Medicine
Pathogenesis
Cell Communication
Pathology and Laboratory Medicine
Biochemistry
Mice
Biopolymers
Cell Movement
Microbial Physiology
Gene expression
Medicine and Health Sciences
Myeloid Cells
Genome Sequencing
lcsh:Science
Regulation of gene expression
0303 health sciences
Multidisciplinary
Paraffin Embedding
Burkholderia thailandensis
biology
Polyhydroxyalkanoates
Temperature
Genomics
Functional Genomics
Infectious Diseases
Host-Pathogen Interactions
Transcriptome Analysis
Research Article
Cell signaling
Burkholderia
Virulence
Real-Time Polymerase Chain Reaction
Microbiology
Molecular Genetics
03 medical and health sciences
Bacterial Proteins
Phagocytosis
Genetics
Animals
Gene Regulation
Model organism
Molecular Biology Techniques
Sequencing Techniques
Gene
Molecular Biology
030304 developmental biology
Biology and life sciences
030306 microbiology
ved/biology
Sequence Analysis
RNA
lcsh:R
Computational Biology
Reproducibility of Results
Gene Expression Regulation
Bacterial
Sequence Analysis
DNA
RNA stability
biology.organism_classification
Genome Analysis
Oxygen
Emerging Infectious Diseases
RAW 264.7 Cells
RNA
lcsh:Q
Genome Expression Analysis
Genome
Bacterial
Zdroj: PLoS ONE, Vol 9, Iss 3, p e93009 (2014)
PLoS ONE
PloS one 9 (2014). doi:10.1371/journal.pone.0093009
info:cnr-pdr/source/autori:Peano, Clelia; Chiaramonte, Fabrizio; Motta, Sara; Pietrelli, Alessandro; Jaillon, Sebastien; Rossi, Elio; Consolandi, Clarissa; Champion, Olivia L.; Michell, Stephen L.; Freddi, Luca; Falciola, Luigi; Basilico, Fabrizio; Garlanda, Cecilia; Mauri, Pierluigi; De Bellis, Gianluca; Landini, Paolo/titolo:Gene and Protein Expression in Response to Different Growth Temperatures and Oxygen Availability in Burkholderia thailandensis/doi:10.1371%2Fjournal.pone.0093009/rivista:PloS one/anno:2014/pagina_da:/pagina_a:/intervallo_pagine:/volume:9
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0093009
Popis: Burkholderia thailandensis, although normally avirulent for mammals, can infect macrophages in vitro and has occasionally been reported to cause pneumonia in humans. It is therefore used as a model organism for the human pathogen B. pseudomallei, to which it is closely related phylogenetically. We characterized the B. thailandensis clinical isolate CDC2721121 (BtCDC272) at the genome level and studied its response to environmental cues associated with human host colonization, namely, temperature and oxygen limitation. Effects of the different growth conditions on BtCDC272 were studied through whole genome transcription studies and analysis of proteins associated with the bacterial cell surface. We found that growth at 37 degrees C, compared to 28 degrees C, negatively affected cell motility and flagella production through a mechanism involving regulation of the flagellin-encoding fliC gene at the mRNA stability level. Growth in oxygen-limiting conditions, in contrast, stimulated various processes linked to virulence, such as lipopolysaccharide production and expression of genes encoding protein secretion systems. Consistent with these observations, BtCDC272 grown in oxygen limitation was more resistant to phagocytosis and strongly induced the production of inflammatory cytokines from murine macrophages. Our results suggest that, while temperature sensing is important for regulation of B. thailandensis cell motility, oxygen limitation has a deeper impact on its physiology and constitutes a crucial environmental signal for the production of virulence factors.
Databáze: OpenAIRE
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