Effects of bevacizumab (Avastin®) on the electroretinogram of isolated rat retina
| Autor: | Michel Doly, David Cia, Nathalie Jacquemot, Jacques Cluzel |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Male
medicine.medical_specialty genetic structures Bevacizumab Angiogenesis Inhibitors Rat retina Antibodies Monoclonal Humanized Retina Cellular and Molecular Neuroscience Antibodies monoclonal Ophthalmology Internal medicine medicine Electroretinography Animals Rats Wistar medicine.diagnostic_test biology business.industry Antibodies Monoclonal General Medicine eye diseases Sensory Systems Rats medicine.anatomical_structure Endocrinology Monoclonal Models Animal biology.protein sense organs Antibody business Erg medicine.drug |
| Zdroj: | Ophthalmic research. 46(3) |
| ISSN: | 1423-0259 |
| Popis: | Aim: To evaluate the in vitro effects of bevacizumab (Avastin®) on the electroretinogram (ERG) in rats using a model of isolated perfused retina ERG recording. Methods: Retinas were isolated from rat eyes and placed in a chamber continuously perfused with a nutrient solution. The ERG was recorded every 3 min. Once the ERG b-wave amplitude was at a steady state, bevacizumab was added at concentrations of 0.25 and 0.5 mg/ml to the perfusion medium for 3 h. Results: We observed no effect on ERG amplitudes or kinetics when bevacizumab was added to the perfusion medium. In addition, we found no significant differences in the survival curves of the b-wave and PIII wave during the application of bevacizumab between bevacizumab-exposed retinas and control retinas. Conclusions: We demonstrate that bevacizumab has no in vitro toxic effects on the ERG of isolated perfused rat retina. Our study supports the retinal safety of bevacizumab with respect to retinal function. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|