Effects of crude kerosene on testosterone levels, aggression and toxicity in rat

Autor: Dinah J. Sawe, Geoffrey K. Maiyoh, Rachel W. Njoroge, Benson N. Macharia
Jazyk: angličtina
Předmět:
medicine.medical_specialty
ALT
alanine transaminase
Health
Toxicology and Mutagenesis
LFT
liver function tests
Hematocrit
Toxicology
Placebo
Bromocresol green (PubChem CID: 6451)
Article
RDW
red cell distribution width
chemistry.chemical_compound
Formaldehyde (PubChem CID: 712)
lcsh:RA1190-1270
AST
aspartate transaminase
T
testosterone
Testosterone (PubChem CID: 6013)
Internal medicine
Ethylenediaminetetraacetic acid (PubChem CID: 6049)
medicine
ELISA
enzyme linked immunosorbent assay
Picrate (PubChem CID: 62496)
Testosterone
HCT
hematocrit concentration
lcsh:Toxicology. Poisons
Creatinine
medicine.diagnostic_test
ALP
alkaline phosphatase
Toxicity
Stomach
RBC
red blood cells
Albumin
EDTA
ethylenediaminetetraacetate
Eosin (PubChem CID: 11048)
Red blood cell distribution width
Creatinine (PubChem CID: 588)
RFT
renal function tests
Crude kerosene
Sex drive
Aggression
Endocrinology
medicine.anatomical_structure
chemistry
Gastritis
Hematoxylin (PubChem CID: 442514)
WBC
white blood cell
Zdroj: Toxicology Reports
Toxicology Reports, Vol 2, Iss C, Pp 175-183 (2015)
ISSN: 2214-7500
DOI: 10.1016/j.toxrep.2014.11.017
Popis: Highlights • Kerosene supplementation increases serum testosterone levels in rat. • Increased testosterone levels were associated with increased aggression. • Kerosene supplementation had varied effects on blood parameters, notably, increased WBC counts. • Supplementation resulted in active/chronic gastritis in the stomach of our rat model.
The use of crude kerosene as a dietary supplement in boarding schools has been a common practice in east Africa and other countries for many years, with the belief of it reducing the sex drive (libido) at the pubertal stage. There is however no scientific basis for this belief. The present study aimed at using a rat animal model to investigate the effects of crude kerosene on serum testosterone levels, aggression and its possible toxic effects. Fifteen male albino rats of approximately similar age and average weights were put into three groups of five animals each; the control group (placebo), low kerosene dose (10 μl/day) group and high kerosene dose (300 μl/day) group. ELISA was used to determine the serum testosterone levels. During treatment, changes in aggression were observed and noted. Liver toxicity was determined using enzyme assays, total protein and albumin while renal toxicity was monitored using serum creatinine levels. A full hemogram was conducted to determine hematological effects. Various tissue biopsies were obtained and examined using histopathological techniques for evidence of toxicity. Contrary to the common belief, our findings showed an overall increase of serum testosterone levels of up to 66% in the low dose and 75% in the high dose groups, with an increasing trend by the end of the study. The high dose group showed significantly increased levels of white blood cells (WBC) (p = 0.036), red blood cells (RBC) (p = 0.025), hematocrit (HCT) (p = 0.03), red cell distribution width (p = 0.028) and platelets (p = 0.017). The histological results of the stomach indicated chronic gastritis.
Databáze: OpenAIRE
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