Influence of MMP inhibitor GM6001 loading of fibre coated polypropylene meshes on wound healing
| Autor: | H. P. Alizai, Gabriele Böhm, Karl-Heinz Heffels, Ursula Mirastschijski, Reinhild Schnabel, Ulf P. Neumann, Jürgen Groll, Nicole Heussen, Peter Josef Ink |
|---|---|
| Přispěvatelé: | Surgery, RS: FHML non-thematic output |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine HUMAN-SKIN Hernia Matrix metalloproteinase inhibitor medicine.medical_treatment Nanofibers sP(EO-stat-PO) GROIN HERNIA Polypropylene mesh Matrix metalloproteinase Matrix (biology) matrix metalloproteinase inhibitor GM6001 Rats Sprague-Dawley chemistry.chemical_compound Drug Delivery Systems 0302 clinical medicine INGUINAL-HERNIA Coated Materials Biocompatible Polylactic Acid-Polyglycolic Acid Copolymer MYOFIBROBLAST FORMATION GROWTH-FACTORS Chemistry Dipeptides Hernia repair Matrix Metalloproteinase 9 030220 oncology & carcinogenesis Matrix Metalloproteinase 2 medicine.symptom neovascularization Myofibroblast poly(lactic-co-glycolic acid) nanofibre Biomedical Engineering Neovascularization Physiologic Inflammation Matrix Metalloproteinase Inhibitors Polypropylenes matrix metalloproteinase-2 Biomaterials 03 medical and health sciences matrix metalloproteinase-9 medicine Animals Herniorrhaphy electrospinning Wound Healing ENVIRONMENT Abdominal Wall INFLAMMATORY RESPONSE Surgical Mesh MATRIX-METALLOPROTEINASE INHIBITION COLLAGEN 030104 developmental biology sense organs IMPLANTATION Wound healing Biomedical engineering hernia repair |
| Zdroj: | Journal of Biomaterials Applications, 32(10), 1343-1359. SAGE Publications Ltd |
| ISSN: | 0885-3282 |
| DOI: | 10.1177/0885328218759043 |
| Popis: | Polypropylene meshes are standard for hernia repair. Matrix metalloproteinases play a central role in inflammation. To reduce the inflammatory response and improve remodelling with an associated reduction of hernia recurrence, we modified polypropylene meshes by nanofibre coating and saturation with the broad-spectrum matrix metalloproteinase inhibitor GM6001. The aim was to modulate the inflammatory reaction, increase collagen deposition and improve mesh biointegration. Polypropylene meshes were surface-modified with star-configured NCO-sP(EO -stat-PO) and covered with electrospun nanofibres (polypropylene-nano) and GM6001 (polypropylene-nano-GM). In a hernia model, defects were reconstructed with one of the meshes. Inflammation, neovascularization, bio-integration, proliferation and apoptosis were assessed histologically, collagen content and gelatinases biochemically. Mesh surface modification resulted in higher inflammatory response compared to polypropylene. Pro-inflammatory matrix metalloproteinase-9 paralleled findings while GM6001 reduced matrix metalloproteinase-9 significantly. Significantly increased matrix metalloproteinase-2 beneficial for remodelling was noted with polypropylene-nano-meshes. Increased vascular endothelial growth factor, neo-vascularization and collagen content were measured in polypropylene-nano-meshes compared to polypropylene. GM6001 significantly reduced myofibroblasts. This effect ended after d14 due to engineering limitations with release of maximal GM6001 loading. Nanofibre-coating of polypropylene-meshes confers better tissue vascularization to the cost of increased inflammation. This phenomenon can be only partially compensated by GM6001. Future research will enable higher GM6001 uptake in nano-coated meshes and may alter mesh biointegration in a more pronounced way. |
| Databáze: | OpenAIRE |
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