Laboratory Evolution of a Saccharomyces cerevisiae × S. eubayanus Hybrid Under Simulated Lager-Brewing Conditions
Autor: | Alex N. Salazar, Line H. Kristensen, Nick Brouwers, Liset Jansen, Jack T. Pronk, Arthur R. Gorter de Vries, Maaike A. Voskamp, Marcel van den Broek, Jean-Marc Daran, Aafke C. A. van Aalst, Thomas Abeel |
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Jazyk: | angličtina |
Rok vydání: | 2019 |
Předmět: |
0301 basic medicine
lcsh:QH426-470 domestication Saccharomyces cerevisiae Genome laboratory evolution Loss of heterozygosity maltotriose utilization Domestication 03 medical and health sciences 0302 clinical medicine flocculation Genetics Copy-number variation Genetics (clinical) Hybrid Original Research Saccharomyces pastorianus biology business.industry technology industry and agriculture Laboratory evolution Chromosome food and beverages biology.organism_classification lcsh:Genetics 030104 developmental biology 030220 oncology & carcinogenesis Brewing Molecular Medicine business |
Zdroj: | Frontiers in Genetics, 10 Frontiers in Genetics Frontiers in Genetics, Vol 10 (2019) |
ISSN: | 1664-8021 |
DOI: | 10.3389/fgene.2019.00242 |
Popis: | Saccharomyces pastorianus lager-brewing yeasts are domesticated hybrids of S. cerevisiae x S. eubayanus that display extensive inter-strain chromosome copy number variation and chromosomal recombinations. It is unclear to what extent such genome rearrangements are intrinsic to the domestication of hybrid brewing yeasts and whether they contribute to their industrial performance. Here, an allodiploid laboratory hybrid of S. cerevisiae and S. eubayanus was evolved for up to 418 generations on wort under simulated lager-brewing conditions in six independent sequential batch bioreactors. Characterization of 55 single-cell isolates from the evolved cultures showed large phenotypic diversity and whole-genome sequencing revealed a large array of mutations. Frequent loss of heterozygosity involved diverse, strain-specific chromosomal translocations, which differed from those observed in domesticated, aneuploid S. pastorianus brewing strains. In contrast to the extensive aneuploidy of domesticated S. pastorianus strains, the evolved isolates only showed limited (segmental) aneuploidy. Specific mutations could be linked to calcium-dependent flocculation, loss of maltotriose utilization and loss of mitochondrial activity, three industrially relevant traits that also occur in domesticated S. pastorianus strains. This study indicates that fast acquisition of extensive aneuploidy is not required for genetic adaptation of S. cerevisiae × S. eubayanus hybrids to brewing environments. In addition, this work demonstrates that, consistent with the diversity of brewing strains for maltotriose utilization, domestication under brewing conditions can result in loss of this industrially relevant trait. These observations have important implications for the design of strategies to improve industrial performance of novel laboratory-made hybrids. |
Databáze: | OpenAIRE |
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