Selection of mutations that alter the osmotic control of transcription of the Salmonella typhimurium proU operon
| Autor: | D G Overdier, V J Prange, L N Csonka, J Druger-Liotta |
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| Rok vydání: | 1987 |
| Předmět: |
Salmonella typhimurium
Proline Transcription Genetic Genetic Linkage Operon Recombinant Fusion Proteins Repressor Locus (genetics) Biology Microbiology Bacterial Proteins Transcription (biology) Genes Regulator Transcriptional regulation Allele Molecular Biology Gene Genetics Regulation of gene expression Genetic Complementation Test Chromosome Mapping Water-Electrolyte Balance Gene Expression Regulation Mutation bacteria Research Article |
| Zdroj: | Journal of Bacteriology. 169:2449-2459 |
| ISSN: | 1098-5530 0021-9193 |
| DOI: | 10.1128/jb.169.6.2449-2459.1987 |
| Popis: | We isolated 60 independent mutations, designated osmX, in Salmonella typhimurium that result in constitutive expression of the normally osmoregulated proU operon. Each of the osmX mutations is closely linked to the proU locus and cis-dominant over the osmX+ allele in diploid strains. These results suggest that the mutations are probably in the 5' transcriptional control region of the proU operon. Our failure to obtain either recessive or unlinked mutations that altered the osmotic control of transcription of the proU operon suggests that transcriptional regulation of the gene is not under the negative control of a repressor protein that is dispensable for cell viability. We discuss possible models for the mechanism of osmotic regulation of transcription of the proU operon. |
| Databáze: | OpenAIRE |
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