Multimodal Imaging Mass Spectrometry to Identify Markers of Pulmonary Arterial Hypertension in Human Lung Tissue Using MALDI-ToF, ToF-SIMS, and Hybrid SIMS
| Autor: | Morad K. Nakhleh, Marc Humbert, Nicolas Elie, Alain Brunelle, Quentin P. Vanbellingen, Julia Zakel, Alexander Pirkl, David Touboul, Pierre Chaurand, Maria-Rosa Ghigna, Elie Fadel, Sylvia Cohen-Kaminsky, Marceau Quatredeniers, Jean-Pierre Le Caer, Sébastien J. Dumas, Sebastiaan Van Nuffel |
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| Přispěvatelé: | Brunelle, Alain, Un grand défi pour l'imagerie par spectrométrie de masse - - DEFIMAGE2015 - ANR-15-CE29-0007 - AAPG2015 - VALID, Idex Paris-Saclay - - IPS2011 - ANR-11-IDEX-0003 - IDEX - VALID, Institut de Chimie des Substances Naturelles (ICSN), Institut de Chimie du CNRS (INC)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), INSERM UMR_S 999 «Pulmonary Hypertension: Pathophysiology and Novel Therapies», Hôpital Marie Lannelongue, 92350 Le Plessis-Robinson, France, IONTOF GmbH, Université de Montréal (UdeM), Laboratoire d'Archéologie Moléculaire et Structurale (LAMS), Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de Chimie du CNRS (INC)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), ANR-15-CE29-0007,DEFIMAGE,Un grand défi pour l'imagerie par spectrométrie de masse(2015), ANR-11-IDEX-0003,IPS,Idex Paris-Saclay(2011), University of Montreal [Montréal, Canada], Institut de Chimie du CNRS (INC)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS) |
| Rok vydání: | 2020 |
| Předmět: |
PTEN
Pathology medicine.medical_specialty [CHIM.ANAL] Chemical Sciences/Analytical chemistry IMAGES Early detection Spectrometry Mass Secondary Ion 010402 general chemistry Diagnostic tools Mass spectrometry 01 natural sciences Mass spectrometry imaging Analytical Chemistry Human lung [CHIM.ANAL]Chemical Sciences/Analytical chemistry NANOPARTICLES medicine SUBLIMATION Humans CYCLE MULTIVARIATE-ANALYSIS Multimodal imaging Pulmonary Arterial Hypertension Chemistry 010401 analytical chemistry 3. Good health 0104 chemical sciences Secondary ion mass spectrometry medicine.anatomical_structure RESOLUTION Potential biomarkers Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization |
| Zdroj: | Analytical Chemistry Analytical Chemistry, 2020, 92 (17), pp.12079. ⟨10.1021/acs.analchem.0c02815⟩ Analytical Chemistry, American Chemical Society, 2020, 92 (17), pp.12079. ⟨10.1021/acs.analchem.0c02815⟩ Analytical Chemistry, 92(17), 12079-12087. American Chemical Society |
| ISSN: | 1520-6882 0003-2700 |
| Popis: | International audience; Pulmonary arterial hypertension (PAH) is a rare and deadly disease affecting roughly 15–60 people per million in Europe with a poorly understood pathology. There are currently no diagnostic tools for early detection nor does a curative treatment exist. The lipid composition of arteries in lung tissue samples from human PAH and control patients were investigated using matrix-assisted laser desorption ionization (MALDI) imaging mass spectrometry (IMS) combined with time-of-flight secondary ion mass spectrometry (TOF-SIMS) imaging. Using random forests as an IMS data analysis technique, it was possible to identify the ion at m/z 885.6 as a marker of PAH in human lung tissue. The m/z 885.6 ion intensity was shown to be significantly higher around diseased arteries and was confirmed to be a diacylglycerophosphoinositol PI(C18:0/C20:4) via MS/MS using a novel hybrid SIMS instrument. The discovery of a potential biomarker opens up new research avenues which may finally lead to a better understanding of the PAH pathology and highlights the vital role IMS can play in modern biomedical research. |
| Databáze: | OpenAIRE |
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