Systemic and Renal Dynamics of Free Sulfhydryl Groups during Living Donor Kidney Transplantation
| Autor: | Nora A. Spraakman, Annemieke M. Coester, Arno R. Bourgonje, Vincent B. Nieuwenhuijs, Jan-Stephan F. Sanders, Henri G. D. Leuvenink, Harry van Goor, Gertrude J. Nieuwenhuijs-Moeke |
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| Přispěvatelé: | Groningen Institute for Organ Transplantation (GIOT), Groningen Kidney Center (GKC) |
| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
PLASMA
Organic Chemistry ischemia-reperfusion injury free thiols kidney transplantation General Medicine Kidney THIOLS Catalysis Computer Science Applications Inorganic Chemistry ischemia–reperfusion injury oxidative stress redox Living Donors Humans Sulfhydryl Compounds REACTIVE OXYGEN Physical and Theoretical Chemistry REPERFUSION INJURY Molecular Biology Spectroscopy |
| Zdroj: | International Journal of Molecular Sciences, 23(17):9789. MDPI AG International Journal of Molecular Sciences; Volume 23; Issue 17; Pages: 9789 |
| ISSN: | 1422-0067 |
| Popis: | During ischemia–reperfusion injury (IRI), reactive oxygen species are produced that can be scavenged by free sulfhydryl groups (R-SH, free thiols). In this study, we hypothesized that R-SH levels decrease as a consequence of renal IRI and that R-SH levels reflect post-transplant graft function. Systemic venous, arterial, renal venous, and urinary samples were collected in donors and recipients before, during, and after transplantation. R-SH was measured colorimetrically. Systemic arterial R-SH levels in recipients increased significantly up to 30 sec after reperfusion (p < 0.001). In contrast, renal venous R-SH levels significantly decreased at 5 and 10 min compared to 30 sec after reperfusion (both p < 0.001). This resulted in a significant decrease in delta R-SH (defined as the difference between renal venous and systemic arterial R-SH levels) till 30 sec after reperfusion (p < 0.001), indicating a net decrease in R-SH levels across the transplanted kidney. Overall, these results suggest trans-renal oxidative stress as a consequence of IRI during kidney transplantation, reflected by systemic and renal changes in R-SH levels in transplant recipients. |
| Databáze: | OpenAIRE |
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