Demonstration of the oligoclonality of an enteropathy associated T-cell lymphoma by monoclonal antibodies and PCR analysis of the T-cell receptor V-beta repertoire on fixed tissue
| Autor: | Una D O'Shea, Kevin M Hollowood, Arthur W. Boylston |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
CD3
T cell Receptors Antigen T-Cell chemical and pharmacologic phenomena Biology Lymphoma T-Cell Polymerase Chain Reaction Pathology and Forensic Medicine T-Lymphocyte Subsets medicine Humans Enteropathy Aged Gene Rearrangement T-cell receptor Antibodies Monoclonal hemic and immune systems T lymphocyte Gene rearrangement medicine.disease Virology Molecular biology Ileal Neoplasms medicine.anatomical_structure biology.protein Enteropathy-associated T-cell lymphoma Female CD8 |
| Zdroj: | Human Pathology. 27:509-513 |
| ISSN: | 0046-8177 |
| DOI: | 10.1016/s0046-8177(96)90095-7 |
| Popis: | The apparent clonality of T cells present in enteropathy associated T cell lymphomas (EATCLs) has been previously reported by showing T cell receptor (TCR) gene rearrangement in fresh tumor tissue. The EATCL presented here exhibits the novel phenotype CD3+, HML-1+, CD4+, CD8+, and TCR Vbeta 8+. The oligoclonality of the tumor cells is shown using the polymerase chain reaction (PCR) on cDNA from RNA extracted from formalin-fixed paraffin-embedded tissue. The T cells present in the lymphoma were predominantly TCR Vbeta 8+. |
| Databáze: | OpenAIRE |
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