Eryptosis in Haemochromatosis: Implications for rheology
| Autor: | Jeanette N. du Plooy, Janette Bester, Etheresia Pretorius |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Adult Male Programmed cell death Physiology Eryptosis Intestinal absorption Flow cytometry 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Physiology (medical) medicine Humans Microscopy Confocal biology medicine.diagnostic_test Chemistry Calpain Hematology Phosphatidylserine Middle Aged Flow Cytometry Molecular biology 030104 developmental biology Apoptosis 030220 oncology & carcinogenesis biology.protein Hydroxyl radical Female Hemochromatosis Cardiology and Cardiovascular Medicine Rheology Intracellular |
| Zdroj: | Clinical hemorheology and microcirculation. 69(4) |
| ISSN: | 1875-8622 |
| Popis: | Background Haemochromatosis is an iron-storage disease with different genetic mutations, characterized by an increased intestinal absorption of iron, resulting in a deposition of excessive amounts of iron in parenchymal cells. When the iron is released in the blood, it is left in an unliganded form, where it can participate in Haber-Weiss and Fenton reactions, creating hydroxyl radicals. Erythrocytes (RBCs) are particularly vulnerable to hydroxyl radical damage, which can result in eryptosis (programmed cell death similar to apoptosis). Study design and methods Here, we used flow cytometry to study the presence of eryptosis in the main genotypic variations of HFE (heterozygous and homozygous C282Y; H63D; C282Y/H63D). We also viewed RBCs from the different mutations using super-resolution Airyscan confocal microscopy. Results Flow cytometry showed significant changes in membrane biochemistry, indicated by the presence of phosphatidylserine (PS) proteins on the outer leaflet of the membrane, as well as increased intracellular calpain. This was found in all of the studied mutations. Airyscan fluorescence revealed PS flip and also microparticles from RBCs. Such microparticles are known to be pro-inflammatory. Conclusion We conclude that RBC pathology is present in all the studied HFE mutations, even in low penetrance mutations, and this might affect rheology in these individuals. |
| Databáze: | OpenAIRE |
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