Involvement of MexS and MexEF-OprN in Resistance to Toxic Ion Chelators in Pseudomonas putida KT2440
| Autor: | Tom Baldow, Yat Kei Lo, Andreas Brachmann, Heinrich Jung, Tania Henríquez, Dina Weydert |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Microbiology (medical)
Operon Mutant medicine.disease_cause Microbiology Article 03 medical and health sciences bipyridyls Virology Pseudomonas medicine lcsh:QH301-705.5 030304 developmental biology 0303 health sciences second site revertant biology 030306 microbiology Pseudomonas aeruginosa Chemistry biology.organism_classification Pseudomonas putida Complementation lcsh:Biology (General) Efflux RND transporter Bacteria |
| Zdroj: | Microorganisms Microorganisms, Vol 8, Iss 1782, p 1782 (2020) Volume 8 Issue 11 |
| ISSN: | 2076-2607 |
| Popis: | Bacteria must be able to cope with harsh environments to survive. In Gram-negative bacteria like Pseudomonas species, resistance-nodulation-division (RND) transporters contribute to this task by pumping toxic compounds out of cells. Previously, we found that the RND system TtgABC of Pseudomonas putida KT2440 confers resistance to toxic metal chelators of the bipyridyl group. Here, we report that the incubation of a ttgB mutant in medium containing 2,2&rsquo bipyridyl generated revertant strains able to grow in the presence of this compound. This trait was related to alterations in the pp_2827 locus (homolog of mexS in Pseudomonas aeruginosa). The deletion and complementation of pp_2827 confirmed the importance of the locus for the revertant phenotype. Furthermore, alteration in the pp_2827 locus stimulated expression of the mexEF-oprN operon encoding an RND efflux pump. Deletion and complementation of mexF confirmed that the latter system can compensate the growth defect of the ttgB mutant in the presence of 2,2&rsquo bipyridyl. To our knowledge, this is the first report on a role of pp_2827 (mexS) in the regulation of mexEF-oprN in P. putida KT2440. The results expand the information about the significance of MexEF-OprN in the stress response of P. putida KT2440 and the mechanisms for coping with bipyridyl toxicity. |
| Databáze: | OpenAIRE |
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