Synthesis of Albumin with Exogenous Mouse-Liver Messenger RNA in a Homologous Cell-Free System
| Autor: | A. J. Faber, T. Tamaoki, S. H. Miall |
|---|---|
| Rok vydání: | 1974 |
| Předmět: |
Five-prime cap
Time Factors Immunoprecipitation Biology Mice Albumins Protein biosynthesis Animals RNA Messenger Polyacrylamide gel electrophoresis Messenger RNA Cell-Free System Albumin RNA General Medicine Ribosomal RNA Molecular biology Kinetics Liver Biochemistry RNA Ribosomal Spectrophotometry Polyribosomes Protein Biosynthesis Electrophoresis Polyacrylamide Gel Spectrophotometry Ultraviolet Ribosomes |
| Zdroj: | Canadian Journal of Biochemistry. 52:429-432 |
| ISSN: | 0008-4018 |
| DOI: | 10.1139/o74-064 |
| Popis: | RNA extracted from total and membrane-bound polysomes of mouse liver was capable of directing protein synthesis in a homologous cell-free system in the presence of a 0.5 M KCl ribosomal wash fraction. Analysis of the products by polyacrylamide gel electrophoresis and immunoprecipitation showed that newly formed albumin could account for up to 8% of the total protein synthesized. |
| Databáze: | OpenAIRE |
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