Effect of upstream reading frames on translation efficiency in simian virus 40 recombinants
| Autor: | Suresh Subramani, Paul Berg, D S Peabody |
|---|---|
| Rok vydání: | 1986 |
| Předmět: |
Recombination
Genetic Genetics Base Sequence biology viruses Single-Strand Specific DNA and RNA Endonucleases Reading frame Translation (biology) DNA Simian virus 40 Cell Biology Endonucleases Ribosome Molecular biology Tetrahydrofolate Dehydrogenase Eukaryotic translation Start codon Protein Biosynthesis parasitic diseases Dihydrofolate reductase Protein biosynthesis biology.protein Coding region Molecular Biology Research Article |
| Zdroj: | Molecular and Cellular Biology. 6:2704-2711 |
| ISSN: | 1098-5549 0270-7306 |
| DOI: | 10.1128/mcb.6.7.2704 |
| Popis: | In a previous report (S. Subramani, R. Mulligan, and P. Berg, Mol. Cell. Biol. 1:854-864, 1981), it was shown that mouse dihydrofolate reductase (DHFR) could be efficiently expressed from simian virus 40 recombinant viruses containing the DHFR cDNA in different locations in the viral late region. This was true even in the case of the SVGT7dhfr26 recombinant, which had the DHFR coding sequence 700 to 800 nucleotides from the 5' end of the mRNA, where it was preceded by the VP2 and VP3 initiator AUGs and a number of other noninitiator AUGs. To investigate the process of internal translation initiation in mammalian cells, we constructed a series of SVGT7dhfr recombinants in which the upstream VP2 and VP3 reading frame was terminated in various positions relative to the DHFR initiation codon. The efficient production of DHFR in infected CV1 cells depended on having the terminators of the VP2-VP3 reading frame positioned upstream or nearby downstream from the DHFR initiation codon. These results reinforce the notion that mammalian ribosomes are capable of translational reinitiation. |
| Databáze: | OpenAIRE |
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