Microevolutionary analysis of the nematode genus Pristionchus suggests a recent evolution of redundant developmental mechanisms during vulva formation
| Autor: | Jagan Srinivasan, Isabel Schlak, Hanh Witte, Andre Pires-daSilva, Min Zheng, Arturo Gutierrez, Ralf J. Sommer, Benno Jungblut |
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| Rok vydání: | 2001 |
| Předmět: |
Male
Nematoda Sequence analysis Molecular Sequence Data Zoology Biology DNA Ribosomal Polymerase Chain Reaction Vulva Evolution Molecular Species Specificity Genus Sequence Homology Nucleic Acid Animals Cell Lineage Mating Ecology Evolution Behavior and Systematics Crosses Genetic Phylogeny Polymorphism Genetic Base Sequence Strain (biology) fungi Gene Expression Regulation Developmental biology.organism_classification Nematode Evolutionary developmental biology Amplified fragment length polymorphism Female Restriction fragment length polymorphism Developmental Biology |
| Zdroj: | ResearcherID |
| ISSN: | 1520-541X |
| Popis: | 1The first three authors contributed equally to this work. *Author for correspondence (email: ralf.sommer@tuebingen.mpg.de) SUMMARY To identify the mechanisms by which molecular variation is introduced into developmental systems, microevolutionary approaches to evolutionary developmental biology have to be taken. Here, we describe the molecular and developmental characterization of laboratory strains of the nematode genus Pristionchus , which lays a foundation for a microevolutionary analysis of vulva development. We describe 13 laboratory strains of the Pristionchus genus that are derived from natural isolates from around the world. Mating experiments and ITS sequence analysis indicated that these 13 strains represent four different species: the gonochoristic species P. lheritieri and three hermaphroditic species, P. pacificus , P. maupasi , and an as yet undescribed species Pristionchus sp., respectively. P. pacificus is represented by five different strains isolated from California, Washington, Hawaii, Ontario, and Poland. Developmental differences during vulva formation are observed between strains from different species but also between strains of P. pacificus , like the strains from California and Poland. In particular, redundant developmental mechanisms present during vulva formation in P. pacificus var. California are absent in other strains. Amplified restriction fragment length polymorphism (AFLP) analyses of the P. pacificus strains revealed that the American strains are highly polymorphic. In contrast, the developmentally distinct strain from Poland is identical to the Californian strain, suggesting that the developmental differences rely on a small number of changes in developmental control genes rather than the accumulation of changes at multiple loci. |
| Databáze: | OpenAIRE |
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