DOMINO1, a member of a small plant-specific gene family, encodes a protein essential for nuclear and nucleolar functions
| Autor: | Martine Devic, Georges Pelletier, Ching-Ming Cheng, Nicole Bechtold, Michel Delseny, Sylvie Albert, Jocelyne Guilleminot, Sylvie Lahmy |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Nucleolus
Molecular Sequence Data Mutant Arabidopsis Ribosome biogenesis Plant Science Biology Ribosome Genetics Gene family Amino Acid Sequence Gene Conserved Sequence Plant Proteins Cell Nucleus Sequence Homology Amino Acid Wild type food and beverages Cell Biology biology.organism_classification Cell biology Fertilization Multigene Family Sequence Alignment Cell Nucleolus |
| Zdroj: | The Plant Journal. 39:809-820 |
| ISSN: | 1365-313X 0960-7412 |
| DOI: | 10.1111/j.1365-313x.2004.02166.x |
| Popis: | Arabidopsis embryos carrying the domino1 mutation grow slowly in comparison with wild type embryos and as a consequence reach only the globular stage at desiccation. The primary defect of the mutation at the cellular level is the large size of the nucleolus that can be observed soon after fertilization in the nuclei of both the embryo and the endosperm. The ultrastructure of mutant nucleoli is drastically different from wild type and points to a fault in ribosome biogenesis. DOMINO1 encodes a protein, which belongs to a plant-specific gene family sharing a common motif of unknown function, present in the tomato DEFECTIVE CHLOROPLASTS AND LEAVES (LeDCL) protein. Using a GFP protein fusion, we show that DOMINO1 is targeted to the nucleus. We propose that inactivation of DOMINO1 has a negative effect on ribosome biogenesis and on the rate of cell division. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text