Escherichia coli-mycobacteria shuttle vectors for operon and gene fusions to lacZ: the pJEM series
| Autor: | Brigitte Gicquel, Juliano Timm, Eng Mong Lim |
|---|---|
| Rok vydání: | 1994 |
| Předmět: |
Operon
Recombinant Fusion Proteins Genetic Vectors Molecular Sequence Data lac operon Regulatory Sequences Nucleic Acid Microbiology Mycobacterium Plasmid Shuttle vector Multiple cloning site Escherichia coli gal operon Cloning Molecular Promoter Regions Genetic Molecular Biology Genetics biology Base Sequence Mycobacterium smegmatis Promoter Mycobacterium tuberculosis biology.organism_classification Molecular biology Mycobacterium bovis Lac Operon Genes Bacterial Research Article |
| Zdroj: | Journal of bacteriology. 176(21) |
| ISSN: | 0021-9193 |
| Popis: | A series of Escherichia coli-mycobacteria shuttle plasmids for the isolation and study of gene regulatory sequences was constructed. These pJEM vectors contain an efficient transcription terminator and multiple cloning sites and allow either operon or gene fusions to lacZ. By constructing operon fusions with pJEM15, we assessed various previously characterized mycobacterial promoters in the fast-growing species Mycobacterium smegmatis and the slow-growing species M. bovis BCG. Our results suggest that M. smegmatis and M. bovis BCG RNA polymerases do not share the same specificity. To isolate new mycobacterial promoters, an M. tuberculosis DNA library was generated, using pJEM13, and screened in M. smegmatis. Several Lac+ clones were isolated, and the beta-galactosidase activity was measured. |
| Databáze: | OpenAIRE |
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