Why Antibiotic Treatment Is Not Enough for Sepsis Resolution: an Evaluation in an Experimental Animal Model
| Autor: | Radhames E. Lizardo, Tony Reyes, Omar Escobedo, Jonathan Halbach, Stephen W. Bickler, Raul Coimbra, Dennis Hawisher, Andrew W. Wang, David M. Cauvi, Antonio De Maio, Joseph Rosas |
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| Přispěvatelé: | Bäumler, Andreas J |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Imipenem medicine.drug_class Fulminant Immunology Antibiotics Inflammation Biology Medical and Health Sciences Microbiology antibiotics sepsis Sepsis Mice 03 medical and health sciences Cecum medicine Animals Host Response and Inflammation Agricultural and Veterinary Sciences Animal Septic shock Inflammatory and immune system Mortality rate cecal ligation and puncture Hematology Biological Sciences bacterial infections and mycoses medicine.disease Survival Analysis infection Anti-Bacterial Agents Treatment Outcome Good Health and Well Being 030104 developmental biology Infectious Diseases medicine.anatomical_structure inflammation Disease Models Parasitology medicine.symptom medicine.drug |
| Zdroj: | Infection and immunity, vol 85, iss 12 |
| ISSN: | 1098-5522 0019-9567 |
| Popis: | Sepsis remains a major health problem at the levels of mortality, morbidity, and economic burden to the health care system, a condition that is aggravated by the development of secondary conditions such as septic shock and multiple-organ failure. Our current understanding of the etiology of human sepsis has advanced, at least in part, due to the use of experimental animal models, particularly the model of cecum ligation and puncture (CLP). Antibiotic treatment has been commonly used in this model to closely mirror the treatment of human septic patients. However, whether their use may obscure the elucidation of the cellular and molecular mechanisms involved in the septic response is questionable. The objective of the present study was to determine the effect of antibiotic treatment in the outcome of a fulminant model of CLP. Various dosing strategies were used for the administration of imipenem, which has broad-spectrum coverage of enteric bacteria. No statistically significant differences in the survival of mice were observed between the different antibiotic dosing strategies and no treatment, suggesting that live bacteria may not be the only factor inducing septic shock. To further investigate this hypothesis, mice were challenged with sterilized or unsterilized cecal contents. We found that exposure of mice to sterilized cecal contents also resulted in a high mortality rate. Therefore, it is possible that bacterial debris, apart from bacterial proliferation, triggers a septic response and contributes to mortality in this model, suggesting that additional factors are involved in the development of septic shock. |
| Databáze: | OpenAIRE |
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