Sugar−Glycerol Cofermentations by Lactobacillus hilgardii Isolated from Wine
| Autor: | Sergio E. Pasteris, Ana M. Strasser de Saad |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Glycerol
Glycerol kinase Otras Ciencias Biológicas ved/biology.organism_classification_rank.species Glycerol dehydratase Wine Fructose Lactobacillus hilgardii Biology Ciencias Biológicas chemistry.chemical_compound Glycerol Kinase Anaerobiosis Sugar Glycerol-3-Phosphate Dehydrogenase (NAD+) GLYCEROL CATABOLISM ved/biology WINE General Chemistry Aerobiosis Lactic acid Lactobacillus Glucose chemistry Biochemistry Fermentation General Agricultural and Biological Sciences LACTOBACILLUS HILGARDII CIENCIAS NATURALES Y EXACTAS |
| Zdroj: | Journal of Agricultural and Food Chemistry. 57:3853-3858 |
| ISSN: | 1520-5118 0021-8561 |
| DOI: | 10.1021/jf803781k |
| Popis: | Glycerol catabolism was studied in Lactobacillus hilgardii X1B from wine, growing on glycerol and limiting glucose or fructose concentrations in anaerobiosis and microaerophilia. Glycerol consumption occurred simultaneously with sugar use, and it was higher with fructose as a cofermenting sugar in microaerophilia. Enzymatic activities of the glycerol kinase and glycerol dehydratase pathways were detected in both incubation conditions. In anaerobiosis, the main products were lactate, acetate, ethanol, and the intermediary product of the glycerol dehydratase pathway, 3- hydroxypropionaldehyde. However, in microaerophilia, 1,3-propanediol was also detected. In anaerobic glucose + glycerol and fructose + glycerol cultures as in microaerophilic glucose + glycerol cultures, glycerol was degraded mainly through the reductive pathway. However, when L. hilgardii X1B was grown on fructose + glycerol cultures in microaerophilia, glycerol dissimilation occurred mainly via the glycerol kinase way. According to these results, L. hilgardii X1B can degrade glycerol by producing 3-hydroxypropionaldehyde and acetic acid, both undesirable products for wine sensorial quality. © 2009 American Chemical Society. Fil: Pasteris, Sergio Enrique. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina. Universidad Nacional de Tucumán. Facultad de Bioquímica, Química y Farmacia; Argentina Fil: Strasser de Saad, Ana Maria. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Centro de Referencia para Lactobacilos; Argentina |
| Databáze: | OpenAIRE |
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