Transgene traceability in transgenic mice: a bioanalytical approach for potential gene-doping analysis
| Autor: | Marcello Buiatti, Maria Minunni, Annarosa Arcangeli, Patrizia Bogani, Stefano Lazzarano, Maria Michela Spiriti |
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| Rok vydání: | 2011 |
| Předmět: |
Genetically modified mouse
Male Bioanalysis Traceability Transgene Clinical Biochemistry Genetic Vectors Green Fluorescent Proteins Mice Transgenic Computational biology Biology Polymerase Chain Reaction Analytical Chemistry Mice Animal model Gene doping Animals Electrophoresis Gel Two-Dimensional Transgenes General Pharmacology Toxicology and Pharmaceutics Gene Doping in Sports business.industry Gene Transfer Techniques Reproducibility of Results General Medicine Genes erbB-1 Genetic Therapy Biotechnology Transgenesis Mice Inbred C57BL Medical Laboratory Technology Models Animal business |
| Zdroj: | Bioanalysis. 3(22) |
| ISSN: | 1757-6199 |
| Popis: | Background: The World Anti-Doping Agency fears the use of gene doping to enhance athletic performances. Thus, a bioanalytical approach based on end point PCR for detecting markers’ of transgenesis traceability was developed. Results: A few sequences from two different vectors using an animal model were selected and traced in different tissues and at different times. In particular, enhanced green fluorescent protein gene and a construct-specific new marker were targeted in the analysis. To make the developed detection approach open to future routine doping analysis, matrices such as urine and tears as well blood were also tested. Conclusion: This study will have impact in evaluating the vector transgenes traceability for the detection of a gene doping event by non-invasive sampling. |
| Databáze: | OpenAIRE |
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