DNA methylation analysis of the EGR3 gene in patients of schizophrenia
| Autor: | Tsung-Ming Hu, Min-Chih Cheng, Su-Min Tsai, Shih-Hsin Hsu |
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| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine Biology Pathogenesis 03 medical and health sciences 0302 clinical medicine mental disorders medicine Humans Luciferase Promoter Regions Genetic Early Growth Response Protein 3 Gene Biological Psychiatry Genetics DNA Methylation medicine.disease In vitro Psychiatry and Mental health 030104 developmental biology Schizophrenia Regulatory sequence DNA methylation Pyrosequencing Female 030217 neurology & neurosurgery |
| Zdroj: | Psychiatry Research. 251:115-117 |
| ISSN: | 0165-1781 |
| Popis: | DNA methylation has been implicated in the pathogenesis of schizophrenia. EGR3 is considered as a potential candidate gene for schizophrenia. We conducted in vitro DNA methylation reaction, Lucia luciferase activity assay, and pyrosequencing assay to assess the DNA methylation of the EGR3 expression underlying the pathophysiology of schizophrenia. We found that DNA methylation of the putative EGR3 regulatory regions attenuated Lucia luciferase activity. There was no difference in the DNA methylation pattern of EGR3 between in 50 schizophrenic patients and 47 controls. Our data suggest that DNA methylation regulated the expression of EGR3 might not be associated with schizophrenia. |
| Databáze: | OpenAIRE |
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