Lysosomal Acid Hydrolases in Primary Monolayer Cultures Derived from Neonatal Brains Exposed Transplacentally to Ethylnitrosourea
| Autor: | Norman Allen, James A. Swenberg, Harold H. Traurig, Nancy R. Clendenon |
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| Rok vydání: | 1972 |
| Předmět: |
Arylsulfatase A
Hydrolases Acid Phosphatase Cytological Techniques Nitrosourea Compounds General Biochemistry Genetics and Molecular Biology Fetus Pregnancy Culture Techniques Hydrolase Animals Maternal-Fetal Exchange Cells Cultured Carcinogen Glucuronidase Brain Chemistry biology Acid phosphatase Brain Transplacental Molecular biology Rats Hexosaminidases Animals Newborn Biochemistry biology.protein Female Sulfatases Lysosomes Neuroglia Acid hydrolase Ethylnitrosourea |
| Zdroj: | Experimental Biology and Medicine. 141:613-617 |
| ISSN: | 1535-3699 1535-3702 |
| Popis: | SummaryThe effect of transplacental exposure of the fetal rat brain to the carcinogen ethylnitrosourea (ENU) on lysosomal acid hydrolase activities in glial cells has been reported. Primary monolayer cultures of brain cells derived from the brains of newborn rats exposed transplacentally to ENU and from unexposed newborn brains were used to provide concentrated samples of glial cells for hydrolase assay. The hydrolases assayed were β-glucuronidase, N-acetyl-β-D-glucosaminidase, arylsulfatase A and B and acid phosphatase and data were expressed as micromoles of substrate hydrolyzed per hour per milligram of protein. Experimental and control cultures were prepared, incubated and assayed contemporaneously and the ratios between treated and control hydrolase activities were used to evaluate the effect of transplacental ENU exposure.Results showed that there was no effect on the activities of the lysosomal acid hydrolases measured in primary monolayer cultures of brain cells derived from newborn rat brains exp... |
| Databáze: | OpenAIRE |
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