Epigallocatechin-3-gallate added after thawing to frozen dog semen: Effect on sperm parameters and ability to bind to oocytes’ zona pellucida
| Autor: | Marcella Spinaci, Daniele Zambelli, Giovanna Galeati, Diego Bucci, Marco Cunto, Beatrice Gadani |
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| Přispěvatelé: | Bucci D., Cunto M., Gadani B., Spinaci M., Zambelli D., Galeati G. |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Male endocrine system Oocyte Heterologous Semen Cryopreservation Catechin Canine Andrology 03 medical and health sciences 0302 clinical medicine Endocrinology Cryoprotective Agents Dogs medicine Animals Acrosome Zona pellucida Sperm motility reproductive and urinary physiology Zona Pellucida Sperm-Ovum Interactions Sperm-Ovum Interaction 030219 obstetrics & reproductive medicine Chemistry urogenital system Animal Sperm Spermatozoa 030104 developmental biology medicine.anatomical_structure Freezing-thawing Oocytes Animal Science and Zoology Antioxidant Cryoprotective Agent Developmental Biology Semen Preservation |
| Popis: | Dog sperm cryopreservation is gaining importance both in breeding dogs for commercial purposes and for pet animals. Anyway, cryopreservation of mammalian spermatozoa, including dog ones, induces some negative effect on sperm fertility, leading to a lower use of this technique and limiting its widespread use. Therefore, studies to improve the quality of canine semen after cryopreservation could have a relevant impact on both the scientific advancement and the clinical practice. The aim of the present work was to investigate the putative ameliorative effect of Epigallochatechin-3-gallate (EGCG) addition to post thawing medium on dog sperm motility, mitochondrial activity, acrosome integrity and on zona-binding ability (zona binding assay). Spermatozoa were thawed in Tris-fructose-citrate medium supplemented with EGCG (0, 25 and 50 μM) and sperm motility, mitochondrial activity and acrosome integrity were assayed at 0.5, 1.5, 3 and 6 h after post thawing incubation at 37 °C. An aliquot of semen from each treatment group after 1.5 h post thawing incubation was washed and used to perform heterologous (using porcine oocytes) or homologous zona binding assay. The results obtained showed that no significant effect is exerted by EGCG on sperm parameters analysed neither at 0.5, 1.5, 3 or 6 h after thawing excepting for the reduction of the percentage of live cells with active mitochondria at the higher dose at 6 h; furthermore, both homologous or heterologous zona binding ability, was not influenced by EGCG. In conclusion, EGCG supplementation to thawing medium does not improve dog sperm quality or zona binding capacity. |
| Databáze: | OpenAIRE |
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