Expression and Characterization of a GH38 α-Mannosidase from the Hyperthermophile Pseudothermotoga thermarum

Autor:	Xing Yan, Xinling Nie, Qingfei Li, Feng Gao, Pei Liu, Zhongbiao Tan, Hao Shi
Rok vydání:	2022
Předmět:	Bioengineering General Medicine Molecular Biology Applied Microbiology and Biotechnology Biochemistry Biotechnology
Zdroj:	Applied biochemistry and biotechnology.
ISSN:	1559-0291
Popis:	This study focused on the bio-characterization of a GH38 α-mannosidase from the hyperthermophile Pseudothermotoga thermarum DSM 5069. We aimed to successfully express and characterize this thermophilic α-mannosidase and to assess its functional properties. Subsequently, recombinant α-mannosidase PtαMan was expressed in Escherichia coli BL21(DE3) and purified via affinity chromatography, and native protein was verified as a tetramer by size exclusion chromatography. In addition, the activity of α-mannosidase PtαMan was relatively stable at pH 5.0-6.5 and temperatures up to 75 ℃. α-Mannosidase PtαMan was active toward Co
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::6983ffa58a26543cc32ba50ce8fc1729 https://pubmed.ncbi.nlm.nih.gov/36399304 Zobrazit plný text záznamu Full text from SpringerLink