Ebulin l Is Internalized in Cells by Both Clathrin-Dependent and -Independent Mechanisms and Does Not Require Clathrin or Dynamin for Intoxication
| Autor: | Lucía Citores, Alicia Llorente, José Miguel Ferreras, Rosario Iglesias |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Ribosome-inactivating proteins
Health Toxicology and Mutagenesis lcsh:Medicine Apoptosis Toxicology Proteinas Ribosomes - Structure chemistry.chemical_compound Lectins Chlorocebus aethiops Toxins 0303 health sciences Proteins - Synthesis biology Chemistry 030302 biochemistry & molecular biology Clathrin-Coated Vesicles ebulin Cell biology rRNA N-glycosylase Ribosome Inactivating Proteins Type 2 Protein Transport Ricin COS Cells symbols Proteínas inactivadoras de ribosomas Intracellular Dynamins intracellular transport endocrine system Endosome Endocytosis Clathrin Article 03 medical and health sciences symbols.namesake 2302 Bioquímica clathrin dynamin Animals Humans endocytosis 030304 developmental biology Dynamin Ribosome-inactivating protein lcsh:R Golgi apparatus Cell membranes Mutation Proteolysis biology.protein Toxinas lectin ribosome-inactivating protein 3214 Toxicología Intracellular transport HeLa Cells |
| Zdroj: | Toxins Volume 13 Issue 2 Toxins, Vol 13, Iss 102, p 102 (2021) |
| ISSN: | 2072-6651 |
| Popis: | Producción Científica Ebulin l is an A-B toxin, and despite the presence of a B chain, this toxin displays much less toxicity to cells than the potent A-B toxin ricin. Here, we studied the binding, mechanisms of endocytosis, and intracellular pathway followed by ebulin l and compared it with ricin. COS-1 cells and HeLa cells with inducible synthesis of a mutant dynamin (K44A) were used in this study. The transport of these toxins was measured using radioactively or fluorescently labeled toxins. The data show that ebulin l binds to cells to a lesser extent than ricin. Moreover, the expression of mutant dynamin does not affect the endocytosis, degradation, or toxicity of ebulin l. However, the inhibition of clathrin-coated pit formation by acidification of the cytosol reduced ebulin l endocytosis but not toxicity. Remarkably, unlike ricin, ebulin l is not transported through the Golgi apparatus to intoxicate the cells and ebulin l induces apoptosis as the predominant cell death mechanism. Therefore, after binding to cells, ebulin l is taken up by clathrin-dependent and -independent endocytosis into the endosomal/lysosomal system, but there is no apparent role for clathrin and dynamin in productive intracellular routing leading to intoxication. Junta de Castilla y León - (grants BIO39/VA39/14 and BIO/VA17/15) Junta de Castilla y León - (grant VA033G19) |
| Databáze: | OpenAIRE |
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