Biological control of the soft rot bacterium Pectobacterium carotovorum by Bacillus amyloliquefaciens strain Ar10 producing glycolipid-like compounds
| Autor: | Selim Jallouli, Sana Azaiez, Salem Elkahoui, Olfa Tabbene, Ferid Limam, Imen Ben Slimene, Naceur Djébali, Ines Karkouch, Rym Essid |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Bacillus amyloliquefaciens 030106 microbiology Pectobacterium carotovorum Microbial Sensitivity Tests Biology Microbiology Plant Roots 03 medical and health sciences Bacterial soft rot Glycolipid Endophytes Antibacterial agent Plant Diseases Solanum tuberosum Strain (chemistry) food and beverages biology.organism_classification Anti-Bacterial Agents Kinetics Biological Control Agents Glycolipids Antibacterial activity Bacteria |
| Zdroj: | Microbiological research. 217 |
| ISSN: | 1618-0623 |
| Popis: | Four hundred and fifty bacteria were evaluated for antagonistic activity against bacterial soft rot of potato caused by Pectobacterium carotovorum sp strain II16. A strain Ar10 exhibiting potent antagonist activity has been identified as Bacillus amyloliquefaciens on the basis of biochemical and molecular characterization. Cell free supernatant showed a broad spectrum of antibacterial activity against human and phytopathogenic bacteria in the range of 10-60 AU/mL. Incubation of P. carotovorum cells with increasing concentrations of the antibacterial compound showed a killing rate of 94.8 and 96% at MIC and 2xMIC respectively. In addition, the antibacterial agent did not exert haemolytic activity at the active concentration and has been preliminary characterized by TLC and GC–MS as a glycolipid compound. Treatment of potato tubers with strain Ar10 for 72 h significantly reduced the severity of disease symptoms (100 and 85.05% reduction of necrosis deep / area and weight loss respectively). The same levels in disease symptoms severity was also recorded following treatment of potato tubers with cell free supernatant for 1 h. Data suggest that protection against potato soft rot disease may be related to glycolipid production by strain Ar10. The present study affords new alternatives for anti-Pectobacterium carotovorum bioactive compounds against the soft rot disease of potato. |
| Databáze: | OpenAIRE |
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