THE ACTION OF T2 BACTERIOPHAGE GHOSTS ON ESCHERICHIA COLI B
| Autor: | R C French, L Siminovitch |
|---|---|
| Rok vydání: | 1955 |
| Předmět: |
Liquid culture
Immunology General Medicine Biology medicine.disease_cause biology.organism_classification Applied Microbiology and Biotechnology Microbiology Agar plate Bacteriophage Colony formation Infected cell Respiration Escherichia coli Genetics Protein biosynthesis medicine Bacteriophage T4 Bacteriophages Molecular Biology |
| Zdroj: | Canadian Journal of Microbiology. 1:757-774 |
| ISSN: | 1480-3275 0008-4166 |
| DOI: | 10.1139/m55-090 |
| Popis: | A study of the physiological effects of T2 ghosts, prepared by the method of Anderson, has been made. It was found that 75% of the ghosts can be adsorbed to host cells as measured with S35. Of these, only 10 to 35% are killers. The remainder, although adsorbed, do not prevent colony formation. A number of experiments, both in liquid culture and on agar plates, show that the effect of this latter class of particles is a temporary one. They lead to an arrest in bacterial multiplication, to a very marked inhibition of protein synthesis (as measured by S35 uptake and β-galactosidase synthesis), to exclusion of either T2 or T1 phage, to continued respiration as with a phage infected cell, and to P32 uptake approaching that to be expected from the number of cells not killed. At about 80 min., these cells reverse the action of the ghosts and resume all their normal metabolic activities. |
| Databáze: | OpenAIRE |
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