Characterization of a globin-coupled oxygen sensor with a gene-regulating function
| Autor: | Evi Vinck, Paolo Ascenzi, Alessandro Bolli, Roy E. Weber, Sylvia Dewilde, Angela Fago, Liesbet Thijs, Sabine Van Doorslaer, David Hoogewijs, Massimiliano Coletta, Maqsudul Alam, Xuehua Wan, Florin Trandafir, L. Moens |
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| Přispěvatelé: | Thijs, L, Vinck, E, Bolli, A, Trandafir, F, Wan, X, Hoogewijs, D, Coletta, M, Fago, A, Weber, Re, VAN DOORSLAER, S, Ascenzi, Paolo, Alam, M, Moens, L, Dewilde, S. |
| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: |
ferrous ion
molecular cloning Conformational change Time Factors Bacteria Carbonylation Genes Isotherms Ligands Proteins Archaea Globin coupled sensors (GCS) Oxygen sensors bacterial protein carbon monoxide globin heme iron myoglobin protein AvGReg protein AvGReg178 Archaebacterium article Azotobacter vinelandii bacterium binding affinity carbonylation conformational transition detoxification gene control nonhuman nucleotide sequence oxygen dissociation curve oxygen sensing priority journal protein domain protein expression protein function protein purification signal transduction Amino Acid Sequence Bacterial Proteins Escherichia coli Gene Expression Regulation Bacterial Heme Hemeproteins Histidine Iron Kinetics Models Biological Molecular Sequence Data Nitric Oxide Oxygen Protein Structure Tertiary Sequence Homology Amino Acid Spectrum Analysis Raman Sequence Homology Biochemistry chemistry.chemical_compound Models Raman biology Bacterial Amino Acid Myoglobin medicine.drug Protein Structure Stereochemistry Ferrous TheoryofComputation_ANALYSISOFALGORITHMSANDPROBLEMCOMPLEXITY medicine Globin Settore BIO/10 Molecular Biology Spectrum Analysis Cell Biology biology.organism_classification Biological chemistry Gene Expression Regulation Ferric Tertiary |
| Zdroj: | Thijs, L, Vinck, E, Bolli, A, Trandafir, F, Wan, X, Hoogewijs, D, Coletta, M, Fago, A, Weber, R E, Doorslaer, S V, Ascenzi, P, Alam, M, Moens, L & Dewilde, S 2007, ' Characterization of a globin-coupled oxygen sensor with a gene-regulating function ', Journal of Biological Chemistry, vol. 282, no. 52, pp. 37325-37340 . https://doi.org/10.1074/jbc.M705541200 Journal of biological chemistry |
| ISSN: | 0021-9258 |
| DOI: | 10.1074/jbc.M705541200 |
| Popis: | Globin-coupled sensors (GCSs) are multiple-domain transducers, consisting of a regulatory globin-like heme-binding domain and a linked transducer domain(s). GCSs have been described in both Archaea and bacteria. They are generally assumed to bind O2 (and perhaps other gaseous ligands) and to transmit a conformational change signal through the transducer domain in response to fluctuating O2 levels. In this study, the heme-binding domain, AvGReg178, and the full protein, AvGReg of the Azotobacter vinelandii GCS, were cloned, expressed, and purified. After purification, the heme iron of AvGReg178 was found to bind O2. This form was stable over many hours. In contrast, the predominant presence of a bis-histidine coordinate heme in ferric AvGReg was revealed. Differences in the heme pocket structure were also observed for the deoxygenated ferrous state of these proteins. The spectra showed that the deoxygenated ferrous derivatives of AvGReg178 and AvGReg are characterized by a penta-coordinate and hexa-coordinate heme iron, respectively. O2 binding isotherms indicate that AvGReg178 and AvGReg show a high affinity for O2 with P50 values at 20 °C of 0.04 and 0.15 torr, respectively. Kinetics of CO binding indicate that AvGReg178 carbonylation conforms to a monophasic process, comparable with that of myoglobin, whereas AvGReg carbonylation conforms to a three-phasic reaction, as observed for several proteins with bis-histidine heme iron coordination. Besides sensing ligands, in vitro data suggest that AvGReg(178) may have a role in O2-mediated NO-detoxification, yielding metAvGReg(178) and nitrate. |
| Databáze: | OpenAIRE |
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