Diagnostic value of loop‐mediated isothermal amplification assay for hand, foot, and mouth disease

Autor: Li-Quan Zhou, Min-Shan Chen, Zhi-Qing Zhan, Xu-Guang Guo, Ying-Zhou Chen, Ya-Ping Li, Xun-Jie Cao
Rok vydání: 2021
Předmět:
0301 basic medicine
Microbiology (medical)
medicine.medical_specialty
diagnosis
polymerase chain reaction
Clinical Biochemistry
Loop-mediated isothermal amplification
hand foot and mouth disease
Diagnostic accuracy
Sensitivity and Specificity
Hand-foot-and-mouth disease
03 medical and health sciences
0302 clinical medicine
Internal medicine
medicine
Humans
Immunology and Allergy
loop‐mediated isothermal amplification
Statistical analysis
Research Articles
business.industry
Quality assessment
Biochemistry (medical)
Public Health
Environmental and Occupational Health
Retrospective cohort study
Hematology
medicine.disease
Confidence interval
Medical Laboratory Technology
030104 developmental biology
Molecular Diagnostic Techniques
meta‐analysis
030220 oncology & carcinogenesis
Meta-analysis
Hand
Foot and Mouth Disease
business
Nucleic Acid Amplification Techniques
Research Article
Zdroj: Journal of Clinical Laboratory Analysis
ISSN: 1098-2825
0887-8013
4202-0212
Popis: Background Nowadays, hand, foot, and mouth disease (HFMD) has a significant negative impact on children's health, especially in the Asia‐Pacific region. Loop‐mediated isothermal amplification assay (LAMP) is a highly efficient and convenient novel tool. However, its diagnostic accuracy for HFMD is still not clear. Therefore, we conducted a meta‐analysis in order to evaluate the potential of LAMP assay for the diagnosis of HFMD, in which the reference standard was polymerase chain reaction (PCR). Methods A protocol was predetermined (CRD42020212882) in PROSPERO. We retrieved seven databases including PubMed for relevant studies published before October 2020. Articles were included if they compared the diagnostic efficiency of LAMP with PCR for HFMD through detecting clinical samples which was more than 15. Statistical analysis was performed by STATA 15.1 software. Risk of bias and applicability were assessed using Quality Assessment of Diagnostic Accuracy Studies. No funding was used for the study. Results A total of 18 retrospective studies including 2495 samples from China were finally included. Reference standards of them included RT‐PCR and non‐RT‐PCR. The merged sensitivity and specificity with 95% confidence interval (95% CI) were 1.00 (0.97–1.00) and 0.97 (0.88–0.99), respectively. The pooled PLR, NLR, and DOR with 95% CI were 11.17 (5.91–21.11), 0.05 (0.03–0.09), and 538.12 (183.17–1580.83), respectively. The AUC of SROC was 1.00 (95% CI: 0.99–1.00). Conclusion In conclusion, our research revealed high sensitivity and specificity of LAMP in diagnosing HFMD. However, more high‐quality research is required to prove this conclusion.
Our research revealed high sensitivity and specificity of LAMP in diagnosing HFMD. The AUC of SROC was 1.00 (0.99–1.00), indicating the high diagnostic accuracy of LAMP for HFMD.
Databáze: OpenAIRE
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