Diagnostic value of loop‐mediated isothermal amplification assay for hand, foot, and mouth disease
Autor: | Li-Quan Zhou, Min-Shan Chen, Zhi-Qing Zhan, Xu-Guang Guo, Ying-Zhou Chen, Ya-Ping Li, Xun-Jie Cao |
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Rok vydání: | 2021 |
Předmět: |
0301 basic medicine
Microbiology (medical) medicine.medical_specialty diagnosis polymerase chain reaction Clinical Biochemistry Loop-mediated isothermal amplification hand foot and mouth disease Diagnostic accuracy Sensitivity and Specificity Hand-foot-and-mouth disease 03 medical and health sciences 0302 clinical medicine Internal medicine medicine Humans Immunology and Allergy loop‐mediated isothermal amplification Statistical analysis Research Articles business.industry Quality assessment Biochemistry (medical) Public Health Environmental and Occupational Health Retrospective cohort study Hematology medicine.disease Confidence interval Medical Laboratory Technology 030104 developmental biology Molecular Diagnostic Techniques meta‐analysis 030220 oncology & carcinogenesis Meta-analysis Hand Foot and Mouth Disease business Nucleic Acid Amplification Techniques Research Article |
Zdroj: | Journal of Clinical Laboratory Analysis |
ISSN: | 1098-2825 0887-8013 4202-0212 |
Popis: | Background Nowadays, hand, foot, and mouth disease (HFMD) has a significant negative impact on children's health, especially in the Asia‐Pacific region. Loop‐mediated isothermal amplification assay (LAMP) is a highly efficient and convenient novel tool. However, its diagnostic accuracy for HFMD is still not clear. Therefore, we conducted a meta‐analysis in order to evaluate the potential of LAMP assay for the diagnosis of HFMD, in which the reference standard was polymerase chain reaction (PCR). Methods A protocol was predetermined (CRD42020212882) in PROSPERO. We retrieved seven databases including PubMed for relevant studies published before October 2020. Articles were included if they compared the diagnostic efficiency of LAMP with PCR for HFMD through detecting clinical samples which was more than 15. Statistical analysis was performed by STATA 15.1 software. Risk of bias and applicability were assessed using Quality Assessment of Diagnostic Accuracy Studies. No funding was used for the study. Results A total of 18 retrospective studies including 2495 samples from China were finally included. Reference standards of them included RT‐PCR and non‐RT‐PCR. The merged sensitivity and specificity with 95% confidence interval (95% CI) were 1.00 (0.97–1.00) and 0.97 (0.88–0.99), respectively. The pooled PLR, NLR, and DOR with 95% CI were 11.17 (5.91–21.11), 0.05 (0.03–0.09), and 538.12 (183.17–1580.83), respectively. The AUC of SROC was 1.00 (95% CI: 0.99–1.00). Conclusion In conclusion, our research revealed high sensitivity and specificity of LAMP in diagnosing HFMD. However, more high‐quality research is required to prove this conclusion. Our research revealed high sensitivity and specificity of LAMP in diagnosing HFMD. The AUC of SROC was 1.00 (0.99–1.00), indicating the high diagnostic accuracy of LAMP for HFMD. |
Databáze: | OpenAIRE |
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