Covalent attachment of functionalized cardiolipin on a biosensor gold surface allows repetitive measurements of anticardiolipin antibodies in serum
| Autor: | Claudia Baier, Alice Schlichtiger, Peter B. Luppa, Makiko Maruyama, Andrew B. Holmes, Ralf Strasser, Meng-Xin Yin, Ulrich Rant, Markus Thaler |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Time Factors
medicine.drug_class Cardiolipins Surface Properties Molecular Conformation Enzyme-Linked Immunosorbent Assay Biosensing Techniques Monoclonal antibody Ligands Biochemistry Analytical Chemistry chemistry.chemical_compound Antiphospholipid syndrome Microscopy Scanning Tunneling medicine Cardiolipin Beta 2-Glycoprotein I Humans Surface plasmon resonance Chemistry Self-assembled monolayer DNA Surface Plasmon Resonance medicine.disease Antiphospholipid Syndrome Molecular biology Receptor–ligand kinetics Kinetics Models Chemical beta 2-Glycoprotein I Antibodies Anticardiolipin lipids (amino acids peptides and proteins) Gold Biosensor |
| Zdroj: | Analytical and bioanalytical chemistry. 405(1) |
| ISSN: | 1618-2650 |
| Popis: | Antiphospholipid antibodies (aPL) are a relevant serological indicator of antiphospholipid syndrome (APS). A solid-state surface with covalently bound ω-amine-functionalized cardiolipin was established and the binding of β2-glycoprotein I (β2-GPI) was investigated either by use of surface plasmon resonance (SPR) biosensor, by electrically switchable DNA interfaces (switchSENSE) and by scanning tunneling microscopy (STM). STM could clearly visualize the attachment of β2-GPI to the cardiolipin surface. Using the switchSENSE sensor, β2-GPI as specific ligand could be identified by increased hydrodynamic friction. The binding of anti-cardiolipin antibodies (aCL) was detected against the ω-amine-functionalized cardiolipin-modified SPR biosensor (aCL biosensor) using sera from healthy donors, APS patients and syphilis patients. Our results showed that the aCL biosensor is a much more sensitive diagnostic device for APS patients compared to previous methods. The specificity between β2-GPI-dependent autoimmune- and β2-GPI-independent infection-associated types of aPLs was also studied and they can be distinguished by the different binding kinetics and patterns. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink