Automated HPLC analyses of drugs of abuse via direct injection of biological fluids followed by simultaneous solid-phase extraction and derivatization with fluorescence detection
| Autor: | Ira S. Krull, Andre J. Bourque, Binyamin Feibush |
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| Rok vydání: | 1994 |
| Předmět: |
Analyte
Acetonitriles Polymers Potassium Compounds Clinical Biochemistry Acetates Biochemistry Methamphetamine Analytical Chemistry Dioxanes Cartridge chemistry.chemical_compound Phenols Drug Discovery Hydroxides Sodium Hydroxide Solid phase extraction Derivatization Molecular Biology Chromatography High Pressure Liquid Nitrobenzenes Pharmacology Syringe driver Detection limit Fluorenes Autoanalysis Chromatography Chemistry Extraction (chemistry) Temperature Dimethylformamide General Medicine Substance Abuse Detection Amphetamine Reagent Solvents Indicators and Reagents |
| Zdroj: | Biomedical Chromatography. 8:53-62 |
| ISSN: | 1099-0801 0269-3879 |
| DOI: | 10.1002/bmc.1130080203 |
| Popis: | An automated system is described for the simultaneous extraction and derivatization of nucleophilic compounds from various biological media. The method includes the use of a solid-phase reagent containing a 9-fluorenylacetate activated ester. The reagent is based on a controlled pore, polystyrene divinylbenzene support prepared through a silica template procedure. An X-Y-Z robotic arm equipped with a needle is used in conjunction with a syringe pump for aspirating and dispensing samples and standards into the HPLC system. A precolumn cartridge containing the solid-phase reagent is put on-line in place of the fixed-volume injection loop. Injections of biological fluids such as urine or plasma with minimal sample treatment and handling are made directly into this reactor. The analytes are derivatized as they are extracted, allowing virtually unlimited sample volumes to be injected. The polymeric cartridge can be used for up to 100 injections without accruing unacceptable reductions in sensitivity. A detection limit of 500 p.p.t. (parts per trillion) of amphetamine in urine was achieved with this system. |
| Databáze: | OpenAIRE |
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