Morphological changes in vascular and circulating blood cells following exposure to detergent sclerosants
| Autor: | Osvaldo Cooley-Andrade, David D.F. Ma, J W Weisel, David E. Connor, Kurosh Parsi |
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| Rok vydání: | 2015 |
| Předmět: |
Male
0301 basic medicine Rouleaux Phalloidin Detergents Population 030204 cardiovascular system & hematology Sodium Tetradecyl Sulfate 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Fluorescence microscope medicine Humans Platelet education Whole blood education.field_of_study Blood Cells business.industry Endothelial Cells General Medicine Fibroblasts Sclerosing Solutions Molecular biology Sodium tetradecyl sulfate Endothelial stem cell 030104 developmental biology chemistry Female Cardiology and Cardiovascular Medicine business medicine.drug |
| Zdroj: | Phlebology: The Journal of Venous Disease. 31:177-191 |
| ISSN: | 1758-1125 0268-3555 |
| DOI: | 10.1177/0268355515573686 |
| Popis: | Objectives To investigate morphological changes in vascular and circulating blood cells following exposure to detergent sclerosants sodium tetradecyl sulfate and polidocanol. Methods Samples of whole blood, isolated leukocytes, platelets, endothelial cells, and fibroblasts were incubated with varying concentrations of sclerosants. Whole blood smears were stained with Giemsa and examined by light and bright field microscopy. Phalloidin and Hoechst stains were used to analyze cytoplasmic and nuclear morphology by fluorescence microscopy. Endothelial cell and fibroblasts were analyzed by live cell imaging. Results Higher concentrations of sclerosants induced cell lysis. Morphological changes in intact cells were observed at sublytic concentrations of detergents. Low concentration sodium tetradecyl sulfate induced erythrocyte acanthocytosis and macrocytosis, while polidocanol induced Rouleaux formation and increased the population of target cells and stomatocytes. Leukocytes showed swelling, blebbing, vacuolation, and nuclear degradation following exposure to sodium tetradecyl sulfate, while polidocanol induced pseudopodia formation, chromatin condensation, and fragmentation. Platelets exhibited pseudopodia with sodium tetradecyl sulfate and a “fried egg” appearance with polidocanol. Exposure to sodium tetradecyl sulfate resulted in size shrinkage in both endothelial cell and fibroblasts, while endothelial cell developed distinct spindle morphology. Polidocanol induced cytoplasmic microfilament bundles in both endothelial cell and fibroblasts. Patchy chromatin condensation was observed following exposure of fibroblasts to either agent. Conclusion Detergent sclerosants are biologically active at sublytic concentrations. The observed morphological changes are consistent with cell activation, apoptosis, and oncosis. The cellular response is concentration dependent, cell-specific, and sclerosant specific. |
| Databáze: | OpenAIRE |
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