Small rat islets are superior to large islets in in vitro function and in transplantation outcomes
| Autor: | K. Kover, W. V. Moore, P. Y. Tong, S. J. Williams, Ronal R. Macgregor, Lisa Stehno-Bittel |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Blood Glucose
Male endocrine system medicine.medical_specialty endocrine system diseases Cell Survival Physiology Endocrinology Diabetes and Metabolism Islets of Langerhans Transplantation Apoptosis In Vitro Techniques Diabetes Mellitus Experimental Islets of Langerhans Necrosis Oxygen Consumption Insulin-Secreting Cells Physiology (medical) Internal medicine Diabetes mellitus Insulin Secretion Animals Insulin Medicine Experimental surgery Insulin secretion Cell survival geography geography.geographical_feature_category business.industry Islet medicine.disease In vitro Rats Transplantation Transplantation outcomes Glucose Treatment Outcome Endocrinology Glucagon-Secreting Cells Potassium business |
| Zdroj: | American Journal of Physiology-Endocrinology and Metabolism. 290:E771-E779 |
| ISSN: | 1522-1555 0193-1849 |
| DOI: | 10.1152/ajpendo.00097.2005 |
| Popis: | Barriers to the use of islet transplantation as a practical treatment for diabetes include the limited number of available donor pancreata. This project was designed to determine whether the size of the islet could influence the success rate of islet transplantations in rats. Islets from adult rats were divided into two groups containing small (diameter 150 μm) islets. An average pancreas yielded three times more small islets than large. Smaller islets were ∼20% more viable, with large islets containing a scattered pattern of necrotic and apoptotic cells or central core cell death. Small islets in culture consumed twice as much oxygen as large islets when normalized for the same islet equivalents. In static incubation, small islets released three times more insulin under basal conditions than did large islets. During exposure to high glucose conditions, the small islets released four times more insulin than the same islet equivalencies of large islets, and five times more insulin was released by the small islets in response to glucose and depolarization with K+. Most importantly, the small islets were far superior to large islets when transplanted into diabetic animals. When marginal islet equivalencies were used for renal subcapsular transplantation, large islets failed to produce euglycemia in any recipient rats, whereas small islets were successful 80% of the time. The results indicate that small islets are superior to large islets in in vitro testing and for transplantation into the kidney capsule of diabetic rats. |
| Databáze: | OpenAIRE |
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