Optimization of DNA extraction of physic nut (Jatropha curcas) by selecting the appropriate leaf
| Autor: | Einstivina Nuryandani, Edi Prayitno |
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| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: | |
| Zdroj: | Nusantara Bioscience, Vol 3, Iss 1, Pp 1-6 (2011) |
| ISSN: | 2087-3956 2087-3948 |
| Popis: | Prayitno E, Nuryandani E. 2011. Optimization of DNA extraction of physic nut (Jatropha curcas) by selecting the appropriateleaf. Nusantara Bioscience 3: 1-6. Jatropha curcas L. has important roles as renewable source of bioenergy. The problem occurs ondifficult of DNA extraction for its molecular breeding programs. The objectives of this research were to study which leaf best as sourceof DNA extraction. Four accession were used, namely J1 and J2 (Jawa Tengah), S1 (South Sumatra), and S2 (Bengkulu). First, third,fifth, seventh, and yellow leaves for each accession were extracted using modification of Doyle and Doyle (1987) method. Visualizationand comparation with Lambda DNA, Spectrophotometer UV-Vis and cutting DNA with EcoRI enzyme were show quality and quantityof DNA. The result showed that third leaves have sufficient quality and quantity as source of DNA. Third leaves DNA quantity for J1(19.33 AŽÂ¼g/mL), J2 (26.21 AŽÂ¼g/mL), S1 (31.20 AŽÂ¼g/mL), dan S2 (61.03 AŽÂ¼g/mL), and quality for each accession were 1.9063 (J1), 2.0162(J2), 2.0116 (S1), and 2.0856 (S2). Key words: Jatropha curcas, DNA extraction, appropriate, leaf. |
| Databáze: | OpenAIRE |
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