IgE-binding components of cultured human keratinocytes in atopic eczema/dermatitis syndrome and their crossreactivity with Malassezia furfur
| Autor: | Johannes Savolainen, Sirje Peltonen, Kirsti Kalimo, K. Pummi, Outi Kortekangas-Savolainen |
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| Rok vydání: | 2004 |
| Předmět: |
Adult
Keratinocytes Male Allergy Antigens Fungal Urticaria Galectin 3 Immunoblotting Immunology Cross Reactions Immunoglobulin E Epitope Dermatitis Atopic Atopy Epitopes Radioallergosorbent Test Antigen Antibody Specificity Psoriasis medicine Humans Immunology and Allergy Cells Cultured Autoantibodies Malassezia integumentary system medicine.diagnostic_test biology Radioallergosorbent test Atopic dermatitis Middle Aged medicine.disease biology.protein Female |
| Zdroj: | Allergy. 59:168-173 |
| ISSN: | 1398-9995 0105-4538 |
| Popis: | Background: Atopic eczema/dermatitis syndrome (AEDS) patients display immunoglobulin E (IgE) reactivity to several antigens, e.g. saprophytic yeasts as Malassezia furfur. AEDS patients also show IgE autoreactivity towards cells of their own tissue including epidermis. Purpose of the study: The aim of this study was to investigate the IgE autoreactivity of AEDS patients to cultured keratinocytes and to reveal potential crossreacting epitopes in cultured keratinocytes and M. furfur. Material and methods: Serum samples of 27 AEDS patients were analyzed, of these 13 were M. furfur radioallergosorbent test (RAST) positive and 14 negative. Four urticaria, three psoriasis, and seven nonatopic patients were included as controls. The studies were performed by using IgE immunoblotting and immunoblotting inhibition methods. Results: Ten IgE-binding protein bands were detected in cultured human keratinocytes by IgE immunoblotting using sera from adult AEDS patients. Anti-keratinocyte IgE antibodies were more associated with elevated S-IgE level than M. furfur RAST. Clear crossreactivity with M. furfur could not be shown. Conclusions: The possible pathomechanism of anti-keratinocyte IgE antibodies is not due to IgE epitope mimicry of saprophytic yeast and local tissue in AEDS skin. |
| Databáze: | OpenAIRE |
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