Cloning and sequencing of a Clostridium perfringens sialidase gene
| Autor: | Friedrich Lottspeich, Peter Roggentin, Roland Schauer, Bernd Rothe |
|---|---|
| Rok vydání: | 1988 |
| Předmět: |
Clostridium perfringens
Molecular Sequence Data Biophysics Neuraminidase (Clostridium perfringens) Gene sequence Sialidase medicine.disease_cause Biochemistry Homology (biology) Restriction fragment Structural Biology Genetics medicine Escherichia coli Amino Acid Sequence Cloning Molecular Molecular Biology Gene biology Base Sequence Oligonucleotide Structural gene Nucleotide Mapping Cell Biology DNA Restriction Enzymes Molecular biology Genes Genes Bacterial biology.protein Gene expression |
| Zdroj: | FEBS letters. 238(1) |
| ISSN: | 0014-5793 |
| Popis: | Escherichia coli was transformed with pUC vectors containing Sau3A restriction fragments (RF) of Clostridium perfringens DNA. Two clones expressed sialidase activity when assayed with the fluorogenic substrate 4-methylumbelliferyl-α-D- N -acetylneuraminic acid. A synthetic oligonucleotide representing the N-terminus of the expressed enzyme hybridized with the clostridial insert and with a corresponding 2.1 kb Sau3A RF of the C. perfringens genome. The insert reduced to 1.4 kb, which still encoded active sialidase, has been sequenced. The structural gene encodes 382 amino acids representing an M r of 42 770. A hydrophobic leader sequence is absent. Upstream from the initiation codon ATG, a GA-rich region is found and considered as the Shine-Dalgarno sequence. Homology with the N-terminus of the Vibrio cholerae sialidase gene and with viral sialidase sequences was not found. |
| Databáze: | OpenAIRE |
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