Piloting a surveillance system to monitor the global patterns of drug efficacy and the emergence of anthelmintic resistance in soil-transmitted helminth control programs : a Starworms study protocol
| Autor: | Jozef Vercruysse, Ruben Santiago Nicholls, Babacar Faye, Johnny Vlaminck, Abdullah Al Kawsar, Mohamed Jamsheed, Innocent Turate, Jaco J. Verweij, Piet Cools, Shaali Ame, John S. Gilleard, Vanisaveth Viengxay, Pauline Mwinzi, Aya Yajima, Jean Bosco Mbonigaba, Denise Mupfasoni, Eliah Shema, Antonio Montresor, Marco Albonico, Jennifer Keiser, Elias Asuming-Brempong, Bruno Levecke, Mike Y. Osei-Atweneboana, Virak Khieu, Alexei Mikhailova, Nadine Ruijeni, Dung Do Trung, Thipphavanh Chanthapaseuth, Roger K. Prichard, Mohammad Jahirul Karim, Ana Luciañez |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Drug
medicine.medical_specialty media_common.quotation_subject 030231 tropical medicine Medicine (miscellaneous) Biochemistry Genetics and Molecular Biology (miscellaneous) Efficacy Kato-Katz thick smear 03 medical and health sciences Study Protocol single nucleotide polymorphisms 0302 clinical medicine Immunology and Microbiology (miscellaneous) Anthelmintic drug Environmental health egg reduction rate medicine Medicine and Health Sciences 030212 general & internal medicine Anthelmintic media_common Protocol (science) benzimidazoles next generation sequencing business.industry preventive chemotherapy Health Policy Public health Public Health Environmental and Occupational Health Soil-transmitted helminths Articles Faecal egg count reduction test anthelmintic drug resistance Soil-transmitted helminth business loop-mediated isothermal amplification medicine.drug |
| Zdroj: | GATES OPEN RESEARCH Gates Open Research |
| ISSN: | 2572-4754 |
| Popis: | To eliminate soil-transmitted helminth (STH) infections as a public health problem, the administration of benzimidazole (BZ) drugs to children has recently intensified. But, as drug pressure increases, the development of anthelmintic drug resistance (AR) becomes a major concern. Currently, there is no global surveillance system to monitor drug efficacy and the emergence of AR. Consequently, it is unclear what the current efficacy of the used drugs is and whether AR is already present. The aim of this study is to pilot a global surveillance system to assess anthelmintic drug efficacy and the emergence of AR in STH control programs. For this, we will incorporate drug efficacy trials into national STH control programs of eight countries (Bangladesh, Cambodia, Lao PDR, Vietnam, Ghana, Rwanda, Senegal and a yet to be defined country in the Americas). In each country, one trial will be performed in one program implementation unit to assess the efficacy of BZ drugs against STHs in school-aged children by faecal egg count reduction test. Stool samples will be collected before and after treatment with BZs for Kato-Katz analysis and preserved to purify parasite DNA. The presence and frequency of known single nucleotide polymorphisms (SNPs) in the β-tubulin genes of the different STHs will subsequently be assessed. This study will provide a global pattern of drug efficacy and emergence of AR in STH control programs. The results will provide complementary insights on the validity of known SNPs in the ß-tubulin gene as a marker for AR in human STHs as well as information on the technical and financial resources required to set up a surveillance system. Finally, the collected stool samples will be an important resource to validate different molecular technologies for the detection of AR markers or to identify novel potential molecular markers associated with AR in STH. |
| Databáze: | OpenAIRE |
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