Bovine polymorphonuclear leukocyte killing of Tritrichomonas foetus
| Autor: | P R Widders, M K Aydintug, R W Leid |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Male
Blood Bactericidal Activity Neutrophils Immunology Antibodies Protozoan Enzyme-Linked Immunosorbent Assay Balanced salt solution Tritrichomonas foetus Buffers Biology Microbiology Immunoglobulin G chemistry.chemical_compound Staphylococcus epidermidis Animals HEPES Complement System Proteins Hydrogen-Ion Concentration biology.organism_classification Infectious Diseases chemistry Humoral immunity biology.protein Cattle Female Parasitology Antibody Research Article |
| Zdroj: | Infection and Immunity. 61:2995-3002 |
| ISSN: | 1098-5522 0019-9567 |
| DOI: | 10.1128/iai.61.7.2995-3002.1993 |
| Popis: | The role of bovine antibody and complement in bovine neutrophil-mediated killing of Tritrichomonas foetus was investigated. No neutrophil-mediated trichomonacidal activity was detected when Hanks' balanced salt solution, a widely utilized and weakly buffered medium, was used. This lack of neutrophil activity was evident even in the presence of specific bovine antibody and bovine complement. Moreover, the pH of the weakly buffered Hanks' balanced salt solution was observed to fall from pH 7.0 to 5.8 in 4 h at 37 degrees C in the presence of T. foetus. The pH of 5.8 inhibited the bactericidal activity of bovine neutrophils for Staphylococcus epidermidis by 53.2% and may have contributed to the lack of neutrophil-mediated trichomonacidal activity in the weakly buffered salt solution. However, T. foetus was susceptible to bovine neutrophil-mediated destruction when a HEPES (N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic acid)-buffered Hanks' balanced salt solution was used (21.8% killing by neutrophils alone). Neither specific bovine immune serum nor purified immune bovine immunoglobulin G2 alone enhanced bovine neutrophil-mediated killing. When complement-sensitized trichomonads were incubated with bovine neutrophils, killing of T. foetus was observed, a result which represented the additive effects of each treatment. Significant (P < 0.05) killing of trichomonads was observed when antibody- and complement-opsonized trichomonads were exposed to bovine neutrophils (> 70% parasite destruction), an effect which reflected the additive nature of each treatment. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|