Extracellular DNA Provides Structural Integrity to a Micrococcus luteus Biofilm
| Autor: | Adam S. Hayward, Mark Geoghegan, Jamie T. Blakeman, Neil Joseph Lant, Klaus Gori, Ana L. Morales-García, Joy Mukherjee |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
DNA
Bacterial animal structures medicine.medical_treatment 02 engineering and technology Cellulase 010402 general chemistry 01 natural sciences Bacterial Adhesion chemistry.chemical_compound Extracellular polymeric substance Electrochemistry medicine Deoxyribonuclease I General Materials Science Spectroscopy chemistry.chemical_classification Protease biology Hydrolysis Polysaccharides Bacterial Biofilm Surfaces and Interfaces Adhesion 021001 nanoscience & nanotechnology Condensed Matter Physics biology.organism_classification 0104 chemical sciences Micrococcus luteus Enzyme chemistry Biofilms biology.protein Biophysics Extracellular Space 0210 nano-technology DNA |
| Zdroj: | Langmuir. 35:6468-6475 |
| ISSN: | 1520-5827 0743-7463 |
| DOI: | 10.1021/acs.langmuir.9b00297 |
| Popis: | Force spectroscopy was used to show that extracellular DNA (eDNA) has a pre-eminent structural role in a biofilm. The adhesive behavior of extracellular polymeric substances to poly(ethylene terephthalate), a model hydrophobic surface, was measured in response to their degradation by hydrolytic enzymes known for their biofilm dispersion potential: DNaseI, protease, cellulase, and mannanase. Only treatment with DNaseI significantly decreased the adhesive force of the model bacterium Micrococcus luteus with the surface, and furthermore this treatment almost completely eliminated any components of the biofilm maintaining the adhesion, establishing a key structural role for eDNA. |
| Databáze: | OpenAIRE |
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