| Autor: |
Martino, Simona Di, Luca, Gabriele De, Grassi, Ludovica, Federici, Giulia, Alfonsi, Romina, Signore, Michele, Addario, Antonio, Salvo, Laura De, Francescangeli, Federica, Sanchez, Massimo, Tirelli, Valentina, Muto, Giovanni, Sperduti, Isabella, Steno Sentinelli, Costantini, Manuela, Pasquini, Luca, Milella, Michele, Haoui, Mustapha, Simone, Giuseppe, Gallucci, Michele, Maria, Ruggero De, Bonci, Désirée |
| Rok vydání: |
2018 |
| DOI: |
10.6084/m9.figshare.7052726.v1 |
| Popis: |
Figure S8. (A) Representative images of Western blot analysis of pAKT S473 and pERK T202/Y204 proteins in clear renal cancer isolated cells (G3 and G4) and commercial lines [primary tumor 786–0 (786) and metastatic Caki-1 (CK1)]. GAPDH expression was used as internal control (B) Anti-mTOR and VEGFR2 protein staining in two representative samples classified by (ISUP) grading as G3 and G4 cases by immunohistochemistry assay. Microscope used Nikon Eclipse 55i, magnification 20X. (C) Representative images of Western blot analysis of pAKT S473 and pERK T202/Y204 proteins in non-sorted stem serum free clear renal cancer enriched cells (Stem Tot.), EpCAM+/CD146+/CD44+ (Stem+) and triple negative (Stem-) sorted cells vs non-sorted clear renal cancer cells maintained in DMEM-FBS condition (DMEM Tot) and evaluated one week after culture. (D) Two-way unsupervised hierarchical clustering of 18 ccRCC samples for the expression of proteins belonging to the angiogenesis pathway. Highlighted in the yellow box are overexpressed protein commonly shared in samples of patients that underwent progression (red arrows). N1 and M1 samples were excluded from the analysis (E) Receiver operating characteristic (ROC) curve showing sensitivity and specificity of HIF-1 alpha and phospho-mTOR (S2448) protein RPPA expressions in predicting progression. The true positive rate (sensitivity) is plotted in function of the false positive rate (100-specificity). The area under the ROC curve (AUC) represents a measure of how well the HIF-1 alpha and phospho-mTOR (S2448) protein RPPA expressions distinguishes progression group from no progression [0.96 (p |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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