Dietary supplementation of alpha-lipoic acid mitigates the negative effects of heat stress in broilers
| Autor: | Nirvay Sah, Chin N. Lee, Rajesh Jha, Sanjeev Wasti, Birendra Mishra |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Hot Temperature Antioxidant medicine.medical_treatment Biochemistry Antioxidants Poultry Cecum RNA Ribosomal 16S Lactobacillus Medicine and Health Sciences Multidisciplinary Thioctic Acid biology Bird Genetics Chemistry Physics Eukaryota Classical Mechanics 04 agricultural and veterinary sciences medicine.anatomical_structure Vertebrates Physical Sciences Medicine Mechanical Stress Anatomy Research Article Science Ileum Butyrate Carbohydrate metabolism Birds 03 medical and health sciences Animal science Genetics medicine Animals Nutrition Organisms 0402 animal and dairy science Broiler Biology and Life Sciences biology.organism_classification Animal Feed 040201 dairy & animal science Diet Bioavailability Gastrointestinal Tract Thermal Stresses 030104 developmental biology Dietary Supplements Amniotes Chickens Zoology Digestive System Animal Genetics Heat-Shock Response |
| Zdroj: | PLoS ONE PLoS ONE, Vol 16, Iss 7, p e0254936 (2021) |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0254936 |
| Popis: | Heat stress accounts for substantial economic loss in the poultry industry by altering the health and performance of chickens. Alpha-lipoic acid (ALA) is a water and fat-soluble antioxidant which is readily absorbed from the intestine resulting in maximum bioavailability. Moreover, ALA acts as a coenzyme in glucose metabolism and helps generate other antioxidants. Considering these benefits, we hypothesized that dietary supplementation of ALA would help mitigate heat stress in poultry. A total of 72 Day-old broiler chicks were randomly assigned into three treatment groups: no heat stress (NHS), heat stress with basal diet (HS), and heat stress with alpha-lipoic acid (HS+ALA); each treatment group had 6 replicate pens with 4 birds in each pen (n = 24/group). The allocated birds were raised under standard husbandry practices for 3 weeks. After 21 d, birds in the HS and HS+ALA groups were exposed to heat stress (33°C for 8 hours during the day) for 3 weeks, while the NHS group was reared under normal conditions (22–24°C). The HS+ALA group received a basal finisher diet fortified with ALA (500 mg/kg) during the treatment period (22 to 42 d), while other birds were provided with the basal finisher diet. Weekly body weight and feed intake were recorded. The cecum digesta for volatile fatty acids (VFAs) analysis and 16S rRNA sequencing for the gut microbiota analysis; and the ileum tissue samples for histological and gene expression analyses were collected on d 42. Exposure to heat stress decreased (PHSP90, PRDX1, GPX3, SOD2, OCLN, and MUC2 genes and higher (PLactobacillus and Peptostreptococcaceae in the cecum of the ALA group. These results indicate that dietary ALA supplementation effectively mitigates the negative effects of heat stress in broilers by improving the expression of heat-shock, tight-junction, antioxidants, and immune-related genes in the intestine, improving villus structures, increasing concentration of major VFAs, and enriching the beneficial microbiota. |
| Databáze: | OpenAIRE |
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