Determination of 5,10,15,20-tetra-(m-hydroxyphenyl) chlorin in tissues by high performance liquid chromatography

Autor:	Q, Wang, H J, Altermatt, H B, Ris, B E, Reynolds, J C, Stewart, R, Bonnett, C K, Lim
Rok vydání:	1993
Předmět:	Pharmacology Radiation-Sensitizing Agents Methanol Muscles Clinical Biochemistry General Medicine Biochemistry Rats Analytical Chemistry Mice Liver Mesoporphyrins Neoplasms Drug Discovery Animals Humans Dimethyl Sulfoxide Molecular Biology Chromatography High Pressure Liquid Skin
Zdroj:	Biomedical Chromatography. 7:155-157
ISSN:	1099-0801 0269-3879
Popis:	A procedure for the extraction and high performance liquid chromatographic (HPLC) determination of the photodynamic therapeutic agent 5,10,15,20-tetra(m-hydroxyphenyl)chlorin in human, rat and mouse tissues following intravenous administration of the drug is described. The tissue (tumour, skin, muscle and liver) was homogenized and extracted into a mixture of methanol:dimethyl sulphoxide:water (32:8:1 by vol.) containing, 5,10,15,20-tetra(p-hydroxyphenyl)chlorin as the internal standard. The precipitated proteins were removed by centrifugation and the supernatant was separated by reversed phase HPLC on a Hypersil-ODS column with 77% (v/v) acetonitrile in 0.1% trifluoroacetic acid as the mobile phase. The solute was detected with high sensitivity and specificity by a UV-VIS detector set at 423 nm.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::695d4a2801c0cbd5a96a44069dbce75e https://doi.org/10.1002/bmc.1130070311 Zobrazit plný text záznamu Plný text