Virus-Like Plaque Formation in Human Cell Culture by Pseudomonas aeruginosa
| Autor: | Robert T. Christian, Peter P. Ludovici |
|---|---|
| Rok vydání: | 1969 |
| Předmět: |
Time Factors
biology Pseudomonas aeruginosa Neomycin Human cell biology.organism_classification medicine.disease_cause Microbiology General Biochemistry Genetics and Molecular Biology Virus Culture Media HeLa Cell culture Culture Techniques Methods medicine Alcaligenes fecalis Bacteria HeLa Cells medicine.drug |
| Zdroj: | Experimental Biology and Medicine. 131:301-305 |
| ISSN: | 1535-3699 1535-3702 |
| DOI: | 10.3181/00379727-131-33863 |
| Popis: | SummaryA chance contamination in the preparation of HeLa cultures in tubes with an organism identified as P. aeruginosa resulted in the formation of plaques within 24 hr which were indistinguishable from viral plaques. At 48 hr, the medium in such tubes became turbid, revealing the presence of bacteria. Filtration of the contaminated medium through a Selas filter or the addition of neomycin eliminated both plaques and turbidity. Stock strains of P. aeruginosa, P. fluorescens, and Alcaligenes fecalis formed similar plaques on HeLa and fibroblastic-like cell line cultured in antibiotic-free medium, whereas strains of E. coli and S. aureus did not. All human epitheloid and fibroblastic-like cell lines tested developed plaques with P. aeruginosa. A linear relationship was observed between bacterial concentration and plaque counts or the time required for plaques to appear. Bacterial plaques were not formed when newborn calf serum (NBCS) was omitted from the medium. At low concentrations of NBCS (1 and 2%), pl... |
| Databáze: | OpenAIRE |
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